Identification of () That Confers Resistance to a New Isolate P1 (CMV-P1) in Pepper ( spp.).

(CMV) is one of the most devastating phytopathogens of . The single dominant resistance gene, (), that confers resistance to the CMV isolate P0 has been overcome by a new isolate (CMV-P1) after being deployed in pepper () breeding for over 20 years. A recently identified Indian cultivar, "Lam32," displays resistance to CMV-P1. In this study, we show that the resistance in "Lam32" is controlled by a single recessive gene, (). We found that conferred resistance to CMV strains including CMV-Korean, CMV-Fny, and CMV-P1, indicating that provides a broad-spectrum type of resistance. We utilized two molecular mapping approaches to determine the chromosomal location of . Bulked segregant analysis (BSA) using amplified fragment-length polymorphism (AFLP) (BSA-AFLP) revealed one marker, cmvAFLP, located 16 cM from . BSA using the Affymetrix pepper array (BSA-Affy) identified a single-nucleotide polymorphism (SNP) marker (Affy4) located 2.3 cM from on chromosome 8. We further screened a pepper germplasm collection of 4,197 accessions for additional CMV-P1 resistance sources and found that some accessions contained equivalent levels of resistance to that of "Lam32." Inheritance and allelism tests demonstrated that all the resistance sources examined contained . Our result thus provide genetic and molecular evidence that is a single recessive gene that confers to pepper an unprecedented resistance to the dangerous new isolate CMV-P1 that had overcome .