Xiao L, Ha S-B
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0404, USA, , , , , , US.
Plant Cell Rep. 1997 Oct;16(12):874-878. doi: 10.1007/s002990050337.
We have established an efficient genetic transformation system for creeping bentgrass (Agrostis palustris Huds.) using particle bombardment. The transformation was performed using the plasmid pZO1052 which contains the reporter β-glucuronidase (uidA) gene and the selectable marker hygromycin phosphotransferase (hph) gene. Transformed calli and plants were obtained via particle bombardment followed by selection of transformants on medium containing 200 mg/l of hygromycin. An average of 4.6 resistant colonies per bombardment were obtained. Southern analysis confirmed the integration of foreign genes in 19 of 21 putative transformants, indicating that selection by hygromycin was highly effective.
我们利用粒子轰击技术为匍匐翦股颖（Agrostis palustris Huds.）建立了一种高效的遗传转化系统。转化过程使用了质粒pZO1052，该质粒包含报告基因β-葡萄糖醛酸酶（uidA）和选择标记潮霉素磷酸转移酶（hph）基因。通过粒子轰击，然后在含有200 mg/l潮霉素的培养基上筛选转化体，获得了转化愈伤组织和植株。每次轰击平均获得4.6个抗性菌落。Southern分析证实了21个推定转化体中有19个整合了外源基因，表明潮霉素选择非常有效。
