Rival A, Beule T, Barre P, Hamon S, Duval Y, Noirot M
CIRAD-CP/ORSTOM ⋅ Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, BP 5045, F-34032 Montpellier, Cedex 01, France Fax no.: +33-467-54-78-00 E-mail:
Plant Cell Rep. 1997 Oct;16(12):884-887. doi: 10.1007/s002990050339.
Flow cytometric analysis performed on two different crosses of dura×pisifera oil palm gave an accurate estimation of nuclear DNA content. The genome size of Elaeis guineensis was found to be 2C=3.76±0.09 pg and therefore ca. 3.4×10 bp. Embryogenic calli and plants showed the same ploidy level, but the measured 2C DNA values differed significantly. No variation in the ploidy level between three different types of calli originating from foliar explants, namely nodular compact callus, fast-growing callus and friable callus was observed. Since fast-growing callus (FGC), already identified as a source of `mantled' phenotype variants, did not show any difference in their ploidy level, these results are consistent with the hypothesis of an epigenetic origin for this type of somaclonal variant.
对两个不同的油棕硬壳种×薄壳种杂交组合进行的流式细胞术分析，准确估计了核DNA含量。发现油棕的基因组大小为2C = 3.76±0.09皮克，因此约为3.4×10⁹碱基对。胚性愈伤组织和植株显示出相同的倍性水平，但测得的2C DNA值差异显著。未观察到源自叶片外植体的三种不同类型愈伤组织，即结节紧实愈伤组织、快速生长愈伤组织和易碎愈伤组织之间倍性水平的变化。由于已被确定为“披被”表型变异体来源的快速生长愈伤组织（FGC）在倍性水平上没有任何差异，这些结果与这种体细胞克隆变异体的表观遗传起源假说一致。
