Al-Mazrooei S, Bhatti M H, Henshaw G G, Taylor N J, Blakesley D
School of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK, , , , , , GB.
Plant Cell Rep. 1997 Jul;16(10):710-714. doi: 10.1007/s002990050307.
Culture procedures have been developed to facilitate the induction and maintenance of somatic embryogenic tissues in 14 out of 16 tested cultivars of sweet potato [Ipomoea batatas (L.) Lam]. Both the size of the axillary bud explant and the type of auxin were found to be critical for the successful induction of somatic embryogenesis. Of the five auxins screened 2,4-dichlorophenoxyacetic acid 2,4-D and 2,4,5-trichlorophenoxyacetic acid were the most effective, with use of the latter inducing the production of embryogenic tissues in 7 cultivars which responded poorly or not at all to 2,4-D. Procedures for secondary/cyclic embryogenesis, formation of mature embryos and their conversion to plants are also described.
已开发出培养程序，以促进16个甘薯[Ipomoea batatas (L.) Lam]测试品种中的14个品种体细胞胚胎发生组织的诱导和维持。发现腋芽外植体的大小和生长素类型对于体细胞胚胎发生的成功诱导至关重要。在筛选的五种生长素中，2,4-二氯苯氧乙酸（2,4-D）和2,4,5-三氯苯氧乙酸最有效，使用后者可在7个对2,4-D反应不佳或根本无反应的品种中诱导产生胚性组织。还描述了次生/循环胚胎发生、成熟胚形成及其转化为植株的程序。
