Bhatti M H, Percival T, Davey C D M, Henshaw G G, Blakesley D
School of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK E-mail:
Plant Cell Rep. 1997 Sep;16(11):802-806. doi: 10.1007/s002990050324.
Embryogenic tissue of nine sweet potato [Ipomoea batatas (L.) Lam] genotypes from Asia, Africa and the Americas was established from in vitro axillary buds on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. Embryogenic aggregates, 1.0-2.0 mm in diameter, were encapsulated in alginate gel, precultured on medium containing elevated levels of sucrose and dehydrated prior to rapid freezing in liquid nitrogen. The maximum survival of embryogenic tissue ranged from 4% to 38%, depending on the genotype. With the incorporation of a slow-cooling step, survival was generally much higher than that obtained after rapid freezing alone. Five of eight genotypes tested with this protocol gave survival percentages in excess of 55%, and a further two in excess of 33%, all after evaporative dehydration. The most effective sucrose treatment(s), however, varied with the genotype.
从亚洲、非洲和美洲的9种甘薯[Ipomoea batatas (L.) Lam]基因型的腋芽在添加了2,4-二氯苯氧乙酸或2,4,5-三氯苯氧乙酸的Murashige和Skoog培养基上建立了胚性组织。将直径为1.0 - 2.0毫米的胚性聚集体包裹在藻酸盐凝胶中，在含有高浓度蔗糖的培养基上预培养，然后在液氮中快速冷冻前进行脱水处理。胚性组织的最大存活率在4%至38%之间，具体取决于基因型。加入缓慢冷却步骤后，存活率通常比仅快速冷冻后获得的存活率高得多。用该方案测试的8个基因型中有5个在蒸发脱水后存活率超过55%，另外2个超过33%。然而，最有效的蔗糖处理方式因基因型而异。
