Kaveriappa K M, Phillips L M, Trigiano R N
Department of Ornamental Horticulture and Landscape Design, Institute of Agriculture, The University of Tennessee, 37901-1071, Knoxville, TN, USA.
Plant Cell Rep. 1997 Apr;16(7):485-489. doi: 10.1007/BF01092771.
A method for regenerating whole plants from nodal (axillary bud) cultures of seedlings was developed for flowering dogwood (Cornus florida L.). The seed source significantly influenced the rate of proliferation, although cultures initiated from each of the seven mother trees produced some shoots. Woody plant medium (WPM) was superior to either Murashige and Skoog or Schenk and Hildebrandt basal medium. 6-Benzyladenine (BA) at 2.2 or 4.4 μM stimulated the generation of significantly more useable shoots (≥1 cm) compared to all other concentrations (0.5-22.5 μM tested. Thidiazuron (TDZ) at 0.6 and 1.1 μM supported proliferation, but strongly inhibited shoot elongation. TDZ initiated cultures transferred to medium containing 4.4 μM BA produced usable shoots after five additional subcultures. Shoots generated adventitious roots when exposed to either a 12-h pulse of relatively high concentrations (246-1230 μM or continuous lower concentrations (0.5-49.0 μM of indolebutyric acid (IBA) for longer periods. Microshoots produced the significantly greatest number of roots when subjected to 4.9 μM IBA in WPM over a 4-week period. Whole plants were acclimatized to the laboratory conditions and subsequently to the greenhouse. The methodology described here should be useful in a breeding program by supplying multiple copies of unique, recombinant genotypes.
已开发出一种从开花山茱萸（Cornus florida L.）幼苗的节（腋芽）培养物中再生完整植株的方法。种子来源显著影响增殖率，尽管从七棵母树中的每一棵上起始培养的材料都产生了一些芽。木本植物培养基（WPM）优于Murashige和Skoog培养基或Schenk和Hildebrandt基本培养基。与所有其他测试浓度（0.5 - 22.5 μM）相比，2.2或4.4 μM的6 - 苄基腺嘌呤（BA）刺激产生了显著更多的可用芽（≥1厘米）。0.6和1.1 μM的噻苯隆（TDZ）支持增殖，但强烈抑制芽的伸长。转移到含有4.4 μM BA的培养基中的TDZ起始培养物在另外五次继代培养后产生了可用芽。当暴露于相对高浓度（246 - 1230 μM）的12小时脉冲或连续较低浓度（0.5 - 49.0 μM）的吲哚丁酸（IBA）较长时间时，芽产生了不定根。在WPM中，微芽在4周时间内接受4.9 μM IBA处理时产生的根数量显著最多。完整植株在实验室条件下驯化，随后在温室中驯化。这里描述的方法通过提供独特的重组基因型的多个副本，应在育种计划中有用。
