Caetano-Anollés G, Bassam B J, Gresshoff P M
Plant Molecular Genetics (OHLD), University of Tennessee, Knoxville, 37901-1071.
Biotechnology (N Y). 1991 Jun;9(6):553-7. doi: 10.1038/nbt0691-553.
The surprising finding that amplification of genomic DNA can be directed by only one oligonucleotide primer of arbitrary sequence to produce a characteristic spectrum of short DNA products of varying complexity, was applied as a strategy to detect genetic differences between organisms. This approach, DNA amplification fingerprinting (DAF), does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins. Primers as short as 5 nucleotides in length can produce complex banding patterns that are resolved by polyacrylamide gel electrophoresis and silver staining. Amplification fragment length polymorphisms (AFLPs) were detected between different human individuals as well as between soybean cultivars. It is anticipated that DAF will have wide application for DNA analysis.
一项惊人的发现是，基因组DNA的扩增可以仅由一条任意序列的寡核苷酸引物引导，从而产生具有不同复杂性的短DNA产物的特征谱，这一发现被用作检测生物体之间遗传差异的策略。这种方法，即DNA扩增指纹图谱（DAF），不依赖于克隆或DNA序列信息，并且可以从病毒、细菌、真菌、植物和动物来源的DNA中产生指纹图谱。长度仅为5个核苷酸的引物可以产生复杂的条带模式，通过聚丙烯酰胺凝胶电泳和银染进行分辨。在不同的人类个体之间以及大豆品种之间检测到了扩增片段长度多态性（AFLP）。预计DAF将在DNA分析中得到广泛应用。
