Castillo B, Smith M A L
Department of Natural Resources and Environmental Sciences, University of Illinois, 1201 S. Dorner Drive, 61801, IL, Urbana, USA.
Plant Cell Rep. 1997 Mar;16(6):385-388. doi: 10.1007/BF01146778.
Direct somatic embryogenesis ofBegonia gracilis was achieved from microcultured laminar segments and petioles on Murashige and Skoog medium with 0.5 mg 1 kinetin and 2% coconut water. Somatic embryos were obtained with greater frequency from petiole explants than from leaf blade sections. Under red light (45 μmol m s), approximately 80% of the petiole explants successfully produced somatic embryos but only 30% of the leaf blade sections responded. However, somatic embryos were significantly more abundant on responding lamina explants (60-70 embryos/leaf section) than on petioles (40-50 embryos/petiole). These trends were similar for explants kept in the dark, but overall production was lower. Somatic embryos were produced more quickly (5 weeks) from petioles than from lamina explants (8 weeks). The somatic embryos germinated to produce plantlets and subsequently shoot cultures with the same appearance as the parental clone.
在含有0.5毫克/升激动素和2%椰汁的Murashige和Skoog培养基上，通过对微培养的叶片切段和叶柄进行培养，实现了纤细秋海棠的直接体细胞胚胎发生。从叶柄外植体获得体细胞胚胎的频率高于叶片切段。在红光（45微摩尔·米⁻²·秒⁻¹）下，约80%的叶柄外植体成功产生体细胞胚胎，而叶片切段只有30%有反应。然而，有反应的叶片外植体上的体细胞胚胎明显更多（每片叶切段60 - 70个胚胎），而叶柄上则为（每根叶柄40 - 50个胚胎）。对于置于黑暗中的外植体，这些趋势相似，但总体产量较低。叶柄产生体细胞胚胎的速度更快（5周），而叶片外植体则需要8周。体细胞胚胎萌发产生植株，随后形成与亲本克隆外观相同的芽培养物。
