Stefanov Ivan, Frank Joachim, Gedamu Lashitew, Misra Santosh
Department of Biochemistry and Microbiology, University of Victoria, V8W 3P6, Victoria, British Columbia, Canada.
Department of Biology, University of Calgary, T2N 1N4, Calgary, Alberta, Canada.
Plant Cell Rep. 1997 Feb;16(5):291-294. doi: 10.1007/BF01088283.
Transgenic tobacco plants and calli bearing the bacterialuid A gene under the transcriptional control ofrbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl β-D-g1ucuronide as a substrate for the β-glucuronidase (uidA) reporter enzyme. Therbc S promoter was repressed by high concentrations of cadmium. An induction of themas promoter was seen after cadmium treatment of seedlings but not calli. The activity of the CaMV35S promoter was unaffected by cadmium in both seedlings and calli.
携带在rbcS、mas和CaMV35S启动子转录控制下的细菌uidA基因的转基因烟草植株和愈伤组织，被暴露于不同浓度的镉中。使用对硝基苯基β-D-葡萄糖醛酸作为β-葡萄糖醛酸酶（uidA）报告酶的底物，监测启动子的转录活性。高浓度的镉会抑制rbcS启动子。在镉处理幼苗后可观察到mas启动子的诱导，但在愈伤组织中未观察到。在幼苗和愈伤组织中，CaMV35S启动子的活性均不受镉的影响。
