Bhuyan A K, Pattnaik S, Chand P K
Plant Tissue and Cell Culture Facility, Post-Graduate Department of Botany, Utkal University, Bhubaneswar-751004 Orissa, India, , , , , , IN.
Plant Cell Rep. 1997 Sep;16(11):779-782. doi: 10.1007/s002990050319.
An efficient and reproducible procedure for the large scale propagation of Murraya koenigii (L.) Spreng. (Curry Leaf Tree) is described. High-frequency direct shoot proliferation was induced in intact seedlings of M. koenigii on modified Murashige and Skoog (1962) (MS) medium supplemented with 5.0 mg/l benzyladenine. Shoot buds originated from the region adjacent to the apex of the primary shoot and the epicotyledonary node of the intact seedling. Shoots elongated following transfer to MS medium without plant growth regulators. The shoot-forming capacity of intact seedlings was influenced by explant orientation. Maximum shoot proliferation was obtained when the shoot-forming region was in direct contact with the medium surface or slightly embedded into the medium. Proliferating shoot cultures were established by repeatedly subculturing mother seedlings on fresh medium of the same composition after excising all newly formed shoots. Roots were formed on excised shoots when they were transfered to half-strength MS containing 1.0 mg/l indole-3-butyric acid. Plantlets were acclimatized and established in soil where they exhibited normal growth.
描述了一种用于大叶桃花心木（咖喱叶树）大规模繁殖的高效且可重复的方法。在添加了5.0毫克/升苄基腺嘌呤的改良Murashige和Skoog（1962）（MS）培养基上，大叶桃花心木的完整幼苗中诱导出高频直接芽增殖。芽从完整幼苗的初生芽顶端和上胚轴节相邻区域产生。转移到不含植物生长调节剂的MS培养基后，芽开始伸长。完整幼苗的芽形成能力受外植体方向影响。当芽形成区域直接与培养基表面接触或稍微嵌入培养基中时，可获得最大的芽增殖。通过在切除所有新形成的芽后，将母苗反复继代培养在相同成分的新鲜培养基上，建立增殖芽培养物。当切下的芽转移到含有1.0毫克/升吲哚-3-丁酸的1/2强度MS培养基上时，会形成根。植株在土壤中驯化并定植，在那里它们表现出正常生长。
