Department of Microbiology and Immunology, Otago School of Biomedical Sciences, The University of Otago, Dunedin 9010, New Zealand.
Department of Surgical Sciences, Dunedin School of Medicine, The University of Otago, Dunedin 9010, New Zealand.
J Immunol. 2019 Mar 15;202(6):1871-1884. doi: 10.4049/jimmunol.1801368. Epub 2019 Feb 6.
T cell infiltration of tumors plays an important role in determining colorectal cancer disease progression and has been incorporated into the Immunoscore prognostic tool. In this study, mass cytometry was used to demonstrate a significant increase in the frequency of both conventional CD25FOXP3CD127 regulatory T cells (Tregs) as well as BLIMP-1 Tregs in the tumor compared with nontumor bowel (NTB) of the same patients. Network cluster analyses using SCAFFoLD, VorteX, and CITRUS revealed that an increase in BLIMP-1 Tregs was a single distinguishing feature of the tumor tissue compared with NTB. BLIMP-1 Tregs represented the most significantly enriched T cell population in the tumor compared with NTB. The enrichment of ICOS, CD45RO, PD-1, PDL-1, LAG-3, CTLA-4, and TIM-3 on BLIMP-1 Tregs suggests that BLIMP-1 Tregs have a more activated phenotype than conventional Tregs and may play a role in antitumor immune responses.
肿瘤中的 T 细胞浸润在决定结直肠癌疾病进展方面起着重要作用，并已被纳入免疫评分预后工具。在这项研究中，使用质谱细胞术证明，与同一患者的非肿瘤肠段（NTB）相比，肿瘤中常规的 CD25FOXP3CD127 调节性 T 细胞（Tregs）以及 BLIMP-1 Tregs 的频率均显著增加。使用 SCAFFoLD、VorteX 和 CITRUS 进行的网络聚类分析表明，与 NTB 相比，BLIMP-1 Tregs 的增加是肿瘤组织的唯一显著特征。与 NTB 相比，BLIMP-1 Tregs 是肿瘤中最显著富集的 T 细胞群。ICOS、CD45RO、PD-1、PDL-1、LAG-3、CTLA-4 和 TIM-3 在 BLIMP-1 Tregs 上的富集表明，BLIMP-1 Tregs 具有比常规 Tregs 更活跃的表型，并且可能在抗肿瘤免疫反应中发挥作用。
