Faculty of Medicine of the University of Porto, Porto, Portugal.
Gastroenterology Department, Centro Hospitalar São João, Porto, Portugal.
Sci Rep. 2019 Feb 6;9(1):1523. doi: 10.1038/s41598-018-38378-6.
Anal squamous intraepithelial lesions (ASIL) or anal intraepithelial neoplasia (AIN) are precancerous lesions. microRNAs (miRNAs) have been implicated in cervical carcinogenesis, but have never been assessed in anal precancerous lesions. Our aim was to evaluate the expression of miR-16, miR-20a, miR-150 and miR-155 in several grades of ASIL obtained from high-risk patients, submitted to anal cancer screening from July 2016 to January 2017. Lesions were classified according to the Lower Anogenital Squamous Terminology (LAST) in low-grade (LSIL) and high-grade squamous intraepithelial lesions (HSIL), and the AIN classification in AIN1, AIN2 and AIN3. A hundred and five biopsies were obtained from 60 patients. Ten samples were negative (9.5%), 63 were LSIL (60%) and 32 were HSIL (30.5%) according to the LAST. Twenty seven (26%) were negative for dysplasia, 46 were classified as AIN1 (44%), 14 as AIN2 (13%) and 18 as AIN3 (17%) according to the AIN classification. There was no statistically significant difference in the fold expression of miR-16, miR-20a, miR-150 and miR-155, according to either classification. Although non- significant, there was an increasing trend in the miR-155 fold expression from negative samples to HSIL, with the highest fold expression increase in both LSIL and HSIL compared to the other miRNAs.
分析鳞状上皮内病变（ASIL）或肛门上皮内瘤变（AIN）是癌前病变。microRNAs（miRNAs）已被认为与宫颈癌的发生有关，但从未在肛门癌前病变中进行过评估。我们的目的是评估 miR-16、miR-20a、miR-150 和 miR-155 在高危患者获得的不同级别 ASIL 中的表达，这些患者于 2016 年 7 月至 2017 年 1 月接受了肛门癌筛查。病变根据低级别（LSIL）和高级别鳞状上皮内病变（HSIL）的下生殖道鳞状上皮术语（LAST）进行分类，并根据 AIN 分类分为 AIN1、AIN2 和 AIN3。从 60 名患者中获得了 105 个活检样本。根据 LAST，有 10 个样本为阴性（9.5%），63 个为 LSIL（60%），32 个为 HSIL（30.5%）。根据 AIN 分类，有 27 个（26%）为无发育不良，46 个为 AIN1（44%），14 个为 AIN2（13%），18 个为 AIN3（17%）。miR-16、miR-20a、miR-150 和 miR-155 的 fold 表达根据两种分类均无统计学差异。虽然无统计学意义，但 miR-155 的 fold 表达从阴性样本到 HSIL 呈上升趋势，在 LSIL 和 HSIL 中与其他 miRNA 相比，fold 表达增加最高。
