实验性旋毛虫病肠道期对Toll样受体4（）基因表达的影响
: Impact on the Expression of Toll-like Receptor 4 () Gene During the Intestinal Phase of Experimental Trichinellosis.
作者信息
Wojtkowiak-Giera Agnieszka, Wandurska-Nowak Elżbieta, Derda Monika, Jankowska Wiesława, Jagodziński Paweł P, Hadaś Edward
机构信息
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 61-701 Poznan, Poland.
Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
出版信息
J Vet Res. 2018 Dec 31;62(4):493-496. doi: 10.2478/jvetres-2018-0063. eCollection 2018 Dec.
INTRODUCTION
Toll-like receptors (TLRs) play a key role in the rapid activation of the innate immune response to a variety of pathogens. The aim of this study was to evaluate the effect of infection on the level of expression of the gene in mouse intestines during the intestinal phase of experimental trichinellosis.
MATERIAL AND METHODS
The experimental material consisted of the small and large intestines of BALB/c mice infected with sampled at 4, 8, and 16 days post infection (dpi).
RESULTS
A statistically significant increase was demonstrated in the mRNA level isolated from the infected mice jejunum at 4, 8, and 16 dpi over the uninfected control. Moreover, at 4, 8, and 16 dpi in the jejunum of infected mice, a strong positive reaction for the presence of TLR4 protein compared with that of uninfected mice was observed.
CONCLUSION
Infection with changes the expression of the gene in the small intestine of the mouse host.
引言
Toll样受体（TLRs）在对多种病原体的先天性免疫反应的快速激活中起关键作用。本研究的目的是评估实验性旋毛虫病肠道期感染对小鼠肠道中该基因表达水平的影响。
材料与方法
实验材料包括感染后4天、8天和16天采集的感染旋毛虫的BALB/c小鼠的小肠和大肠。
结果
在感染后4天、8天和16天，从感染小鼠空肠分离的该mRNA水平与未感染对照组相比有统计学意义的显著增加。此外，在感染小鼠空肠的4天、8天和16天，与未感染小鼠相比，观察到TLR4蛋白存在的强阳性反应。
结论
旋毛虫感染改变了小鼠宿主小肠中该基因的表达。
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