Faculty of Science, Biology Department, Arak University, Arak, Iran.
Andrologia. 2019 Jun;51(5):e13242. doi: 10.1111/and.13242. Epub 2019 Feb 6.
Cadmium is known as an oxidative stress-inducing factor. Silymarin extracted from Silybum marianum is regarded as a potent antioxidant. The present study investigated the preventing effects of silymarin on cadmium chloride-induced toxicity in terms of testis histopathology and serum testosterone level as well as oxidative stress indicators in mice. In addition, the activities of antioxidant defence enzymes was evaluated. Adult male mice were divided into four groups (n = 6 in each group): (a) control; (b) cadmium chloride; (c) silymarin + cadmium chloride and (d) Silymarin. In this study, cadmium chloride significantly decreased the diameter and wall thickness of the seminiferous tubule, diameter of the spermatogonia nucleus and serum testosterone levels compared to the control group. Furthermore, in mice treated with this pollutant, a significant increase in malondialdehyde was observed while ferric reducing antioxidant power level, and the activity of catalase, superoxide dismutase and glutathione peroxidase were significantly reduced in the testis. In the silymarin + cadmium chloride group, silymarin could significantly reverse the toxic effects of cadmium chloride. The findings of this study showed that silymarin, as a potent antioxidant, can compensate the adverse effects of cadmium chloride on testis histopathology, testosterone level, oxidative stress indicators and antioxidant defence enzymes in mice.
镉是一种已知的诱导氧化应激的因素。水飞蓟素是从水飞蓟中提取的，被认为是一种有效的抗氧化剂。本研究旨在探讨水飞蓟素对氯化镉诱导的雄性小鼠睾丸组织病理学和血清睾酮水平以及氧化应激指标的毒性的预防作用，同时评估抗氧化防御酶的活性。成年雄性小鼠被分为四组（每组 6 只）：（a）对照组；（b）氯化镉组；（c）水飞蓟素+氯化镉组；（d）水飞蓟素组。在本研究中，与对照组相比，氯化镉显著降低了曲细精管的直径和壁厚、精原细胞核的直径以及血清睾酮水平。此外，在接受这种污染物治疗的小鼠中，丙二醛的水平显著升高，而睾丸中的铁还原抗氧化能力水平以及过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性显著降低。在水飞蓟素+氯化镉组中，水飞蓟素可以显著逆转氯化镉的毒性作用。本研究结果表明，水飞蓟素作为一种有效的抗氧化剂，可以补偿氯化镉对雄性小鼠睾丸组织病理学、睾酮水平、氧化应激指标和抗氧化防御酶的不良影响。
