Zhao Xiaoli, Gao Jinyan, Hogenkamp Astrid, Knippels Leon M J, Garssen Johan, Bai Jing, Yang Anshu, Wu Yong, Chen Hongbing
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, 3584 CG Utrecht, The Netherlands.
Foods. 2021 Oct 22;10(11):2542. doi: 10.3390/foods10112542.
Selenium (Se)-enriched proteins are an important dietary source of Se for humans; however, only a few Se-enriched proteins have been identified. In the present study, we tested for potential antioxidant activity by Se-enriched soy protein, both in vitro and in vivo. Se-enriched soy protein isolate (S-SPI) was shown to have a higher free radical scavenging ability compared to ordinary soy protein isolate (O-SPI). Furthermore, Caco-2 cell viability was improved by S-SPI at low doses, whereas O-SPI did not. In addition, S-SPI was shown to inhibit oxidative stress via modulation of the NRF2-HO1 signaling pathway, upregulating the expression of downstream antioxidant enzymes (GPx, SOD). To further study the antioxidant capacity of S-SPI, BALB/c female mice were given oral gavages with 0.8 mL of S-SPI or O-SPI (5 g/kg/d, 20 g/kg/d and 40 g/kg/d) or saline as control. Hepatic GPx and SOD activity increased with increasing S-SPI dosage, but not with O-SPI. Taken together, our results suggest that Se-enriched soy protein has a high antioxidant ability and may be used as a dietary supplement for people with oxidative dam-age-mediated diseases.
富硒蛋白是人类膳食中硒的重要来源；然而，目前仅鉴定出少数几种富硒蛋白。在本研究中，我们对富硒大豆蛋白在体外和体内的潜在抗氧化活性进行了测试。结果表明，与普通大豆分离蛋白（O-SPI）相比，富硒大豆分离蛋白（S-SPI）具有更高的自由基清除能力。此外，低剂量的S-SPI可提高Caco-2细胞活力，而O-SPI则无此作用。另外，S-SPI可通过调节NRF2-HO1信号通路抑制氧化应激，上调下游抗氧化酶（GPx、SOD）的表达。为进一步研究S-SPI的抗氧化能力，将BALB/c雌性小鼠分为几组，分别口服灌胃0.8 mL的S-SPI或O-SPI（5 g/kg/d、20 g/kg/d和40 g/kg/d），以生理盐水作为对照。肝脏中GPx和SOD的活性随S-SPI剂量的增加而升高，但O-SPI组则无此变化。综上所述，我们的结果表明，富硒大豆蛋白具有较高的抗氧化能力，可作为氧化损伤介导疾病患者的膳食补充剂。
