Faculty of Science, Department of Zoology, Charles University, Praha, Czech Republic.
Faculty of Science, Department of Botany, Charles University, Praha, Czech Republic.
Cytometry A. 2019 Jul;95(7):746-756. doi: 10.1002/cyto.a.23729. Epub 2019 Feb 6.
The human bed bug Cimex lectularius is one of the most prevalent human ectoparasites in temperate climate zones. The cytogenetic features of this resilient pest include holokinetic chromosomes, special chromosome behavior in meiosis, and numerical variation of chromosomes, where the diploid number ranges from 26 + X X Y to 26 + X Y. It is desirable to assess the nuclear DNA content of various cytotypes for a further detailed study of the C. lectularius genome. Detailed knowledge of the DNA content of this parasite could also clarify the origin of additional chromosomes. The average nuclear genome size C. lectularius with 2n = 26 + X X Y is 2C = 1.94 pg for males and 1.95 pg for females. There is a significant correlation between genome size and the number of chromosomes, but in some specimens with additional chromosomes, nuclear genome size decreases or remains average. Several species used as the internal reference standard were tested for further investigations of genome size in C. lectularius, and the plant Solanum pseudocaspicum turned out to be the most suitable. © 2019 International Society for Advancement of Cytometry.
人床虱 Cimex lectularius 是温带气候带中最普遍的人类外寄生虫之一。这种有弹性的害虫的细胞遗传学特征包括全动染色体、减数分裂中的特殊染色体行为以及染色体的数值变异，其中二倍体数范围为 26+XXY 至 26+XY。评估各种细胞型的核 DNA 含量，以便对 C. lectularius 基因组进行进一步的详细研究是可取的。对这种寄生虫 DNA 含量的详细了解还可以澄清额外染色体的起源。具有 2n=26+XXY 的平均核基因组大小 C. lectularius 为雄性的 2C=1.94pg，雌性的为 1.95pg。基因组大小与染色体数量之间存在显著相关性，但在一些具有额外染色体的标本中，核基因组大小减小或保持平均水平。为了进一步研究 C. lectularius 中的基因组大小，测试了几种用作内部参考标准的物种，结果表明植物 Solanum pseudocaspicum 是最合适的。©2019 国际细胞分析促进学会。
