Tolan H K, Tozan-Beceren A, Sardas S, Senkesen O, Celikel C, Gencosmanoglu R, Yegen C
Department of General Surgery, Health Sciences University Umraniye Training and Research Hospital, Umraniye, Istanbul, Turkey.
Department of Toxicology, Marmara University-Faculty of Pharmacy, Istanbul, Turkey.
Niger J Clin Pract. 2019 Feb;22(2):194-200. doi: 10.4103/njcp.njcp_251_18.
Colorectal cancers are third most common cancer in both genders. They are associated with genetic and environmental factors. Staging is important in the prognosis. Carcinoembryonic Antigen (CEA) provides preliminary information and there is a correlation between Proliferation Index (PI) and prognostic variables. Our aim is to investigate the relationship between DNA repair capacity and clinico-pathologic factors.
The blood samples taken from cancer patients were irradiated. DNA repair capacity by comet technique was calculated. The CEA values were recorded. Pathology reports were collected and PI values were calculated. s.
Total of 30 patients; male (n: 14) and female (n: 16) with a median age of 66.37 ± 10.32 were included. Mean CEA value was 42.85 (1.46 - 422.30 μgr/ml) μgr/ml. Mean % DNA repair capacity was 44.49 ± 5.24. In the pathology; 21 (70%) were T3 tumors; 18 (60%) had lymphatic and 12 (40%) had vascular 2 invasion. Perineural invasion was present in 8 (26.7%). According to the proliferation index (PI); 16 (53.3%) were in high percentile (PI > 66%) group.
There was a significant correlation between; perineural invasion and tumor grade (P = 0.043); lymphatic and perineural invasion (P = 0.006); lymphatic invasion and vascular invasion (P = 0.034) and the DNA repair capacity with the lymphatic invasion (P = 0.026). There was also a statistically significant (P = 0.044) relationship between PI and lymphatic invasion. As a result in colorectal cancer patients DNA repair capacity can be used as a biomarker in the staging and also in the prediction of the tumor behavior.
结直肠癌是男女中第三常见的癌症。它们与遗传和环境因素有关。分期对预后很重要。癌胚抗原（CEA）提供初步信息，增殖指数（PI）与预后变量之间存在相关性。我们的目的是研究DNA修复能力与临床病理因素之间的关系。
采集癌症患者的血液样本并进行辐照。通过彗星技术计算DNA修复能力。记录CEA值。收集病理报告并计算PI值。
共纳入30例患者；男性（n = 14）和女性（n = 16），中位年龄为66.37±10.32岁。平均CEA值为42.85（1.46 - 422.30μg/ml）μg/ml。平均DNA修复能力百分比为44.49±5.24。在病理方面；21例（70%）为T3肿瘤；18例（60%）有淋巴侵犯，12例（40%）有血管侵犯。8例（26.7%）存在神经周围侵犯。根据增殖指数（PI）；16例（53.3%）属于高百分位数（PI>66%）组。
神经周围侵犯与肿瘤分级之间存在显著相关性（P = 0.043）；淋巴和神经周围侵犯（P = 0.006）；淋巴侵犯和血管侵犯（P = 0.034）以及DNA修复能力与淋巴侵犯之间存在显著相关性（P = 0.026）。PI与淋巴侵犯之间也存在统计学显著关系（P = 用0.044）。结果表明，在结直肠癌患者中，DNA修复能力可作为分期以及预测肿瘤行为的生物标志物。
