Arifagaoglu O, Oncul S, Ercan A, Olcay O, Ersu B
Department of Prosthodontics, Faculty of Dentistry, Baskent University, Ankara, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Niger J Clin Pract. 2019 Feb;22(2):251-257. doi: 10.4103/njcp.njcp_364_18.
Titanium and its alloys are currently the most common dental implant materials. For the best bone-implant contact, machined titanium is subjected to various surface treatments. In the present study, proliferation of human gingivial fibroblast (HGF-1) cells on Grade 5 titanium disks covered with Grade 23 titanium by selective laser melting technology was evaluated.
The main aim was to provide a novel surface procedure providing more biocompatible external structure with a biomechanically intact inner structure and increasing cell proliferation.
Forty-eight titanium Grade 5 machined disks with 5 mm of diameter was divided into four groups. Group 1, also known as the control group, was not subjected to any surface treatment. Group 2 was treated with sand-blasted, large-grid, acid-etching (SLA) technique and Group 3 was treated with selective laser melting (SLM) method. Group 4 was treated with both SLM and SLA. The surface topography was analyzed using scanning electron microscope and the roughness of the samples was evaluated via optic profilometer. Additionally, optical tensiometer was used to measure the surface wettability. To obtain further insights on biocompability of the samples, HGF-1 cell viability at 48 h was assessed with MTT assay. These results were also confirmed by fluorescent staining. Results: Although Ra value and wettability of Group 4 were the highest amongst the samples handled, based on 48 h MTT results and fluorescent staining, highest cell proliferation was observed in Group 3.
It was verifed that the surface topography, roughness, and wettability are all crucial factors on healthy cell populations. Therefore, it was concluded that disks treated with SLM were shown to express the most suitable condition for biocompability.
钛及其合金是目前最常用的牙科植入材料。为了实现最佳的骨-植入物接触，加工后的钛会进行各种表面处理。在本研究中，评估了通过选择性激光熔化技术在5级钛盘上覆盖23级钛后，人牙龈成纤维细胞（HGF-1）的增殖情况。
主要目的是提供一种新颖的表面处理方法，该方法能提供生物相容性更好的外部结构以及生物力学完整的内部结构，并促进细胞增殖。
将48个直径为5毫米的5级加工钛盘分为四组。第1组，也称为对照组，未进行任何表面处理。第2组采用喷砂、大网格、酸蚀（SLA）技术处理，第3组采用选择性激光熔化（SLM）方法处理。第4组同时采用SLM和SLA处理。使用扫描电子显微镜分析表面形貌，并通过光学轮廓仪评估样品的粗糙度。此外，使用光学张力计测量表面润湿性。为了进一步了解样品的生物相容性，采用MTT法评估48小时时HGF-1细胞的活力。这些结果也通过荧光染色得到证实。结果：尽管在处理的样品中，第4组的Ra值和润湿性最高，但根据48小时的MTT结果和荧光染色，第3组观察到最高的细胞增殖。
证实了表面形貌、粗糙度和润湿性都是影响健康细胞群体的关键因素。因此，得出结论，经SLM处理的盘片显示出最适合生物相容性的条件。
