Department of Oral Implantology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, PR China.
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, PR China.
J Biomater Appl. 2020 Aug;35(2):169-181. doi: 10.1177/0885328220920457. Epub 2020 Apr 27.
Selective laser melting used in manufacturing custom-made titanium implants becomes more popular. In view of the important role played by osteoclasts in peri-implant bone resorption and osseointegration, we modified selective laser melting-manufactured titanium surfaces using sandblasting/alkali-heating and sandblasting/acid-etching, and investigated their effect on osteoclast differentiation as well as their underlying mechanisms. The properties of the surfaces, including elements, roughness, wettability and topography, were analyzed. We evaluated the proliferation and morphology of primary mouse bone marrow-derived monocytes, as well as induced osteoclasts derived from bone marrow-derived monocytes, on samples. Then, osteoclast differentiation was determined by the tartrate-resistant acid phosphatase activity assay, calcitonin receptors immunofluorescence staining and the expression of osteoclast-related genes. The results showed that sandblasting/alkali-heating established nanonet structure with the lowest water contact angle, and both sandblasting/alkali-heating and sandblasting/acid-etching significantly decreased surface roughness and heterogeneity compared with selective laser melting. Surface modifications of selective laser melting-produced titanium altered bone marrow-derived monocyte morphology and suppressed bone marrow-derived monocyte proliferation and osteoclastogenesis in vitro (sandblasting/alkali-heating>sandblasting/acid-etching>selective laser melting). These surface modifications reduced the activation of extracellular signal-regulated kinase and c-Jun N-terminal kinases compared to native-selective laser melting. Sandblasting/alkali-heating additionally blocked tumor necrosis factor receptor-associated factor 6 recruitment. The results suggested that sandblasting/alkali-heating and sandblasting/acid-etching modifications on selective laser melting titanium could inhibit osteoclast differentiation through suppressing extracellular signal-regulated kinase and c-Jun N-terminal kinase phosphorylation in mitogen-activated protein kinase signaling pathway and provide a promising technique which might reduce peri-implant bone resorption for optimizing native-selective laser melting implants.
选择性激光熔化在定制钛植入物制造中的应用变得越来越流行。鉴于破骨细胞在种植体周围骨吸收和骨整合中的重要作用，我们使用喷砂/碱热处理和喷砂/酸蚀对选择性激光熔化制造的钛表面进行了改性，并研究了它们对破骨细胞分化的影响及其潜在机制。分析了表面的特性，包括元素、粗糙度、润湿性和形貌。我们评估了样品上原代小鼠骨髓源性单核细胞的增殖和形态，以及由骨髓源性单核细胞诱导的破骨细胞。然后，通过抗酒石酸酸性磷酸酶活性测定、降钙素受体免疫荧光染色和破骨细胞相关基因的表达来确定破骨细胞分化。结果表明，喷砂/碱热处理形成纳米网结构，水接触角最低，喷砂/碱热处理和喷砂/酸蚀处理均显著降低了表面粗糙度和异质性，与选择性激光熔化相比。选择性激光熔化产生的钛表面改性改变了骨髓源性单核细胞的形态，并抑制了体外骨髓源性单核细胞的增殖和破骨细胞生成（喷砂/碱热处理>喷砂/酸蚀处理>选择性激光熔化）。与原始选择性激光熔化相比，这些表面改性降低了细胞外信号调节激酶和 c-Jun N 末端激酶的激活。喷砂/碱热处理还阻断了肿瘤坏死因子受体相关因子 6 的募集。结果表明，选择性激光熔化钛上的喷砂/碱热处理和喷砂/酸蚀改性可通过抑制丝裂原激活蛋白激酶信号通路中细胞外信号调节激酶和 c-Jun N 末端激酶磷酸化来抑制破骨细胞分化，并提供了一种有前途的技术，该技术可能通过减少种植体周围骨吸收来优化原始选择性激光熔化植入物。
