Electrical & Computer Engineering Department, University of California, Los Angeles, California 90095, USA.
Lab Chip. 2019 Mar 13;19(6):1027-1034. doi: 10.1039/c9lc00011a.
We developed a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers. In this multiplexed vertical flow immunodiagnostic assay (xVFA), a colorimetric signal is generated by gold nanoparticles captured on a spatially-multiplexed sensing membrane containing specific antigens. The assay is completed in 20 minutes, following which the sensing membrane is imaged by a cost-effective mobile-phone reader. The images are sent to a server, where the results are rapidly analyzed and relayed back to the user. The performance of the assay was evaluated by measuring Lyme-specific antibodies in human sera as model target antibodies. The presented platform is rapid, simple, inexpensive, and allows for simultaneous and quantitative measurement of multiple antibodies and/or antigens making it a suitable point-of-care platform for disease diagnostics.
我们开发了一种通过垂直堆叠功能纸层来检测人血清中抗体的多重即时免疫诊断检测方法。在这种多重垂直流动免疫诊断检测（xVFA）中，通过在含有特定抗原的空间多重化传感膜上捕获金纳米颗粒产生比色信号。该检测在 20 分钟内完成，之后通过成本效益高的移动电话读取器对传感膜进行成像。将图像发送到服务器，在那里快速分析结果并反馈给用户。通过测量人血清中的莱姆病特异性抗体作为模型靶抗体来评估该检测方法的性能。所提出的平台快速、简单、廉价，并允许同时定量测量多种抗体和/或抗原，使其成为适合疾病诊断的即时护理平台。
