Kim Min Woo, Park Hong-Je, Park Chan Yeong, Kim Ji Hong, Cho Chae Hwan, Phan Le Minh Tu, Park Jong Pil, Kailasa Suresh Kumar, Lee Chi-Ho, Park Tae Jung
Department of Chemistry, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University 84 Heukseok-ro, Dongjak-gu Seoul 06974 Republic of Korea
R&D Center, Medexx Co., Ltd 5F, #B, Korea Bio Park, 700 Daewangpangyo-ro, Bundang-gu Seongnam-si Gyeonggi-do 13488 Republic of Korea.
RSC Adv. 2020 Aug 12;10(50):29759-29764. doi: 10.1039/d0ra03677c. eCollection 2020 Aug 10.
The detection of bovine viral diarrhea virus (BVDV), which is a pathogen inducing fatal gastrointestinal disease in cattle, is becoming a momentous issue in the livestock farm. In that, BVDV is related to inapparent infection and various diseases with high transmissibility; it has also led to considerable economic losses. In this study, a simple dot-blotting method was devised to construct a rapid screening system for BVDV. Based on the BVDV-specific bioreceptors, it was anchored on the gold nanoparticles (AuNPs) to generate the seeding sites for signaling; then the signals were amplified by adopting the overgrowth of copper nano-polyhedral shells on AuNPs. The developed detection system shows a low detection limit of 4.4 copies per mL, and even this could be distinguished with naked eyes. These results indicate that the designed nanobiosensor possesses not only high sensitivity and selectivity but also potential usage on a point-of-care testing platform for BVDV.
牛病毒性腹泻病毒（BVDV）是一种可引发牛致命性胃肠道疾病的病原体，其检测已成为养殖场的一个重大问题。鉴于BVDV与隐性感染以及多种具有高传播性的疾病相关，它还造成了相当大的经济损失。在本研究中，设计了一种简单的斑点印迹法来构建BVDV快速筛查系统。基于BVDV特异性生物受体，将其锚定在金纳米颗粒（AuNPs）上以产生信号的种子位点；然后通过在AuNPs上生长铜纳米多面体壳来放大信号。所开发的检测系统显示出低至每毫升4.4个拷贝的检测限，甚至这一结果用肉眼也可辨别。这些结果表明，所设计的纳米生物传感器不仅具有高灵敏度和选择性，而且在BVDV即时检测平台上具有潜在用途。
