M.V. Lomonosov Moscow State University, Moscow, 119991, Russia.
A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Moscow, 119991, Russia.
MAGMA. 2019 Jun;32(3):307-315. doi: 10.1007/s10334-019-00739-1. Epub 2019 Feb 7.
To identify the technical aspects of the potential use of clinically approved perfluorodecalin (PFD, CF) for F magnetic resonance imaging (MRI) oximetry method at high magnetic field 7.05 T.
F T measurements were made on a set of PFD samples with different oxygen contents (0%, 21%, and 100%) at room (21 °C) and body temperature (37 °C). In vivo MRI studies were carried out on one healthy rat and two rats with C6 brain glioma.
The selective excitation of the magnetically equivalent F nuclei of CF groups of trans-isomer of PFD, which give a doublet at a frequency of about - 140 ppm (in relation the chemical shift of trifluoroacetic acid, which is - 76.55 ppm) should be done for correct implementation of F MRI oximetry method. The amount of PFD equal to 30 μl is the optimal for obtaining reliable data on the measured T values. In this case, the standard deviation of T does not exceed 5%. In vivo MRI studies showed that the values of the partial pressure of oxygen (pO) decrease from normal values of about 38 mmHg (healthy brain) to almost 0 mmHg at the last stage of tumor growth.
The study showed the feasibility of the successful application of PFD for F MRI oximetry method.
确定在 7.05T 高磁场下临床批准的全氟癸烷（PFD，CF）用于 F 磁共振成像（MRI）血氧计方法的潜在用途的技术方面。
在室温（21°C）和体温（37°C）下，对具有不同氧含量（0%、21%和 100%）的一组 PFD 样品进行 F T 测量。在一只健康大鼠和两只 C6 脑胶质瘤大鼠上进行了体内 MRI 研究。
对于正确实施 F MRI 血氧计方法，应选择性地激发 PFD 的顺式异构体的 CF 基团的磁等价 F 核，该基团在约-140ppm 的频率处给出双峰（相对于三氟乙酸的化学位移，为-76.55ppm）。获得关于测量 T 值的可靠数据的最佳 PFD 量等于 30μl。在这种情况下，T 的标准偏差不超过 5%。体内 MRI 研究表明，氧分压（pO）的值从正常的约 38mmHg（健康大脑）下降到肿瘤生长的最后阶段几乎为 0mmHg。
该研究表明 PFD 成功应用于 F MRI 血氧计方法的可行性。
