Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Gent, Belgium.
MAGMA. 2010 Sep;23(4):217-26. doi: 10.1007/s10334-010-0219-6. Epub 2010 Jun 25.
This study presents a reproducible phantom which mimics oxygen-consuming tissue and can be used for the validation of (19)F MRI oximetry.
The phantom consists of a haemodialysis filter of which the outer compartment is filled with a gelatin matrix containing viable yeast cells. Perfluorocarbon emulsions can be added to the gelatin matrix to simulate sequestered perfluorocarbons. A blood-substituting perfluorocarbon fluid is pumped through the lumen of the fibres in the filter. (19)F relaxometry MRI is performed with a fast 2D Look-Locker imaging sequence on a clinical 3T scanner.
Acute and perfusion-related hypoxia were simulated and imaged spatially and temporally using the phantom.
The presented experimental setup can be used to simulate oxygen consumption by somatic cells in vivo and for validating computational biophysical models of hypoxia, as measured with (19)F MRI oximetry.
本研究提出了一种可复制的仿氧耗组织的体模，可用于（19）F MRI 血氧计的验证。
该体模由一个血液透析过滤器组成，其外腔充满了含有活酵母细胞的明胶基质。可以将全氟碳乳液添加到明胶基质中，以模拟隔离的全氟碳化合物。一种替代血液的全氟碳液体通过过滤器纤维的管腔被泵送。在临床 3T 扫描仪上使用快速 2D Look-Locker 成像序列进行（19）F 弛豫率 MRI。
使用该体模对急性和灌注相关缺氧进行了时空模拟和成像。
所提出的实验设置可用于模拟体内体细胞的耗氧量，并验证（19）F MRI 血氧计测量的缺氧计算生物物理模型。
