Center of Health Research, Førde Hospital Trust, Førde, Norway.
Haukeland University Hospital, Bergen, Norway.
J Hosp Infect. 2019 Aug;102(4):419-424. doi: 10.1016/j.jhin.2019.01.026. Epub 2019 Feb 5.
Hand hygiene plays a vital role in the prevention of transmission of micro-organisms. Ozone (O) is a highly reactive gas with a broad spectrum of antimicrobial effects on bacteria, viruses, and protozoa. It can easily be produced locally in small generators, and dissolved in tap water, and quickly transmits into ordinary O in the surrounding air.
To compare ozonized tap water and alcohol rub in decontamination of bacterially contaminated hands.
A cross-over study among 30 nursing students. Hands were artificially contaminated with Escherichia coli (ATCC 25922), then sanitized with ozonized tap water (0.8 or 4 ppm) or 3 mL standard alcohol-based rub (Antibac 85%). The transient microbes from fingers were cultivated and colony-forming units (cfu)/mL were counted. The test procedure was modified from European Standard EN 1500:2013.
All contaminated hands before disinfection showed cfu >30,000/mL. The mean (SD) bacterial counts in (cfu/mL) on both hands combined were 1017 (1391) after using ozonized water, and 2337 (4664) after alcohol hand disinfection. The median (range) values were 500 (0-6700) and 250 (0-16,000) respectively (non-significant difference). Twenty per cent of participants reported adverse skin effects (burning/dryness) from alcohol disinfection compared with no adverse sensations with ozone.
Ozonized tap water is an effective decontaminant of E. coli, and it could be an alternative to traditional alcohol-fluid hand disinfectants both in healthcare institutions and public places. Ozonized water may be especially valuable for individuals with skin problems.
手部卫生在预防微生物传播方面起着至关重要的作用。臭氧（O）是一种具有广谱抗菌作用的高反应性气体，对细菌、病毒和原生动物都有作用。它可以很容易地在小型发生器中就地产生，并溶解在自来水中，然后迅速在周围空气中转化为普通的 O。
比较臭氧水和酒精擦手液在消毒细菌污染的手部方面的效果。
在 30 名护理学生中进行了一项交叉研究。将大肠杆菌（ATCC 25922）人工污染手部，然后使用臭氧水（0.8 或 4ppm）或 3 毫升标准酒精基擦手液（Antibac 85%）进行消毒。从手指上采集瞬时微生物进行培养，并计算菌落形成单位（cfu）/毫升。测试程序改编自欧洲标准 EN 1500：2013。
所有未经消毒的污染手部在消毒前的 cfu 均>30000/ml。使用臭氧水后，双手结合的平均（SD）细菌计数为 1017（1391）cfu/ml，使用酒精手消毒后为 2337（4664）cfu/ml。中位数（范围）值分别为 500（0-6700）和 250（0-16000）（无显著差异）。20%的参与者报告使用酒精消毒后出现皮肤不良反应（灼热/干燥），而使用臭氧则没有不良反应。
臭氧水是大肠杆菌的有效消毒剂，它可以替代传统的酒精液体手部消毒剂，无论是在医疗机构还是公共场所都可以使用。臭氧水对于有皮肤问题的人可能特别有价值。
