基于一维傅里叶频谱镜操作的无光栅旋转结构光照明显微成像
Structured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum.
作者信息
Jin Xin, Ding Xuemei, Tan Jiubin, Yao Xincheng, Shen Cheng, Zhou Xuyang, Tan Cuimei, Liu Shutian, Liu Zhengjun
出版信息
Opt Express. 2019 Feb 4;27(3):2016-2028. doi: 10.1364/OE.27.002016.
Structured illumination microscopy (SIM) is a rapidly developing a super-resolution optical microscopy technique. With SIM, the grating is needed in order to rotate several angles for illuminating the sample in different directions. Multiple rotations reduce the imaging speed and grating rotation angle errors damage the image recovery quality. We introduce mirror transformation on one-dimension (1D) Fourier spectrum to SIM for resolving the problems of low imaging speed and severe impact on image reconstruction quality by grating rotation angle errors. When mirror operation and SIM are combined, the grating is placed at an orientation for obtaining three shadow images. The three shadow images are acquired by CCD at three different phase shift for a direction of grating. Thus, the SIM imaging speed is faster and the effect on image reconstruction quality by grating rotation angle errors is greatly reduced.
结构光照明显微镜（SIM）是一种快速发展的超分辨率光学显微镜技术。使用SIM时，需要光栅以便旋转多个角度以在不同方向上照射样品。多次旋转会降低成像速度，并且光栅旋转角度误差会损害图像恢复质量。我们将一维（1D）傅里叶频谱上的镜像变换引入到SIM中，以解决成像速度低以及光栅旋转角度误差对图像重建质量的严重影响问题。当将镜像操作与SIM相结合时，将光栅放置在一个方向上以获得三个阴影图像。通过电荷耦合器件（CCD）在光栅的一个方向上以三个不同的相移采集这三个阴影图像。因此，SIM成像速度更快，并且光栅旋转角度误差对图像重建质量的影响大大降低。
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