Won Dong Jae, Seong Ki Seung, Jang Cheol Ho, Lee Jin Sil, Ko Jung A, Bae Hojae, Park Hyun Jin
a Department of Biotechnology , College of Life Science and Biotechnology, Korea University , Seongbuk-gu, Seoul , Republic of Korea.
b Korea Food Research Institute , Wanju-gun, Jeollabuk-do , Republic of Korea.
Biosci Biotechnol Biochem. 2019 May;83(5):942-951. doi: 10.1080/09168451.2019.1576497. Epub 2019 Feb 7.
Bioavailability and bone loss inhibitory effects of vitamin D derived from UV-irradiated shiitake mushroom were determined in vivo. The effect of the absence of ovaries on the bioavailability of vitamin D and bone structure was also investigated. Sham operated (sham) and ovariectomized (OVX) rats were divided in 3 groups according to their diets, i.e. control: only vitamin D-deficient diets; UV(X): vitamin D-deficient diets with non-irradiated mushroom powder; UV(O): vitamin D-deficient diets with irradiated mushroom powder. The obtained results showed that vitamin D from shiitake mushroom was able to increase bone mineral density and trabecular bone structure of femur bone as well as its bioavailability. The absence of estrogen induced adverse effects not only on bioavailability of vitamin D but also on trabecular bone. In conclusion, vitamin D-fortified shiitake mushroom might help postmenopausal women increase vitamin D bioavailability and retard trabecular bone loss. Abbreviations: OVX: ovariectomized; 25(OH)D: 25-hydroxyvitamin D; 1,25(OH)2D: 1,25-dihydroxyvitamin D; BMD: bone mineral density; micro-CT: micro computed tomography; RSM: response surface methodology; RP-HPLC: Reverse phase-high performance liquid chromatography; MS/MS: tandem mass spectrometry; E2: estradiol; NTx: N-terminal telopeptide of type I collagen; BV/TV: bone volume/total volume; BS/BV: bone surface/bone volume; Tb.Th: trabecular thickness; Tb.Sp: trabecular separation.
在体内测定了紫外线照射香菇衍生的维生素D的生物利用度和抑制骨质流失的作用。还研究了卵巢缺失对维生素D生物利用度和骨骼结构的影响。根据饮食将假手术（假手术组）和去卵巢（OVX）大鼠分为3组，即对照组：仅维生素D缺乏饮食；UV(X)组：含未辐照香菇粉的维生素D缺乏饮食；UV(O)组：含辐照香菇粉的维生素D缺乏饮食。所得结果表明，香菇中的维生素D能够增加股骨的骨矿物质密度和小梁骨结构及其生物利用度。雌激素缺乏不仅对维生素D的生物利用度有不利影响，对小梁骨也有不利影响。总之，富含维生素D的香菇可能有助于绝经后妇女提高维生素D生物利用度并延缓小梁骨丢失。缩写：OVX：去卵巢；25(OH)D：25-羟基维生素D；1,25(OH)2D：1,25-二羟基维生素D；BMD：骨矿物质密度；显微CT：显微计算机断层扫描；RSM：响应面法；RP-HPLC：反相高效液相色谱；MS/MS：串联质谱；E2：雌二醇；NTx：I型胶原N端肽；BV/TV：骨体积/总体积；BS/BV：骨表面积/骨体积；Tb.Th：小梁厚度；Tb.Sp：小梁间距。
