Ghaith Doaa M, Mohamed Zeinat K, Farahat Mohamed G, Aboulkasem Shahin Walaa, Mohamed Hadeel O
Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science, Cairo University, Cairo, Egypt.
Arab J Gastroenterol. 2019 Mar;20(1):19-22. doi: 10.1016/j.ajg.2019.01.002. Epub 2019 Feb 4.
Colonized patients with carbapenamase producing Enterobacteriaceae (CPE) are vulnerable to invasive infections from their endogenous flora. We aimed to assess faecal colonization with (CPE) among children admitted to Cairo University paediatric intensive care units (ICUs). The phenotypic and genotypic characterizations of carbapenemase-producing Enterobacteriaceae were also studied.
A total of 413 Enterobacteriaceae isolates have been isolated from cultured rectal swabs of 100 children. All swabs were inoculated on ChromID™ CARBA agar to screen for carbapenem resistant Enterobacteriaceae (CRE). Disk diffusion method, Modified Hodge test (MHT) and further genotypic detection of carbapenemases genes (bla, bla and bla, bla and bla) by multiplex PCR were done.
Out of 413 Enterobacteriaceae isolates; 100 isolates were defined as CRE. Bla was detected in (33%); Escherichia coli (n = 11), Klebsiella oxytoca (n = 3) and Klebsiella pneumoniae (n = 19), while (27%) carried blaEscherichia coli (n = 7), and Klebsiella pneumoniae (n = 20).
Prevalence of carbapenem resistant Enterobacteriaceae was 24%, various genes of carbapenemases were detected in 80% of carbapenem resistant Enterobacteriaceae with dominance of bla. Understanding the colonization status of our patients with strict infection control measures can reduce the risk of horizontal gene transfer of carbapenemases.
携带产碳青霉烯酶肠杆菌科细菌（CPE）的定植患者易受其自身内源性菌群引起的侵袭性感染。我们旨在评估开罗大学儿科重症监护病房（ICU）收治儿童的粪便中CPE定植情况。同时还研究了产碳青霉烯酶肠杆菌科细菌的表型和基因型特征。
从100名儿童的直肠拭子培养物中分离出总共413株肠杆菌科细菌。所有拭子均接种于ChromID™ CARBA琼脂上，以筛选耐碳青霉烯肠杆菌科细菌（CRE）。采用纸片扩散法、改良 Hodge试验（MHT），并通过多重PCR对碳青霉烯酶基因（bla、bla和bla、bla和bla）进行进一步的基因检测。
在413株肠杆菌科细菌分离株中，100株被定义为CRE。在33%的菌株中检测到bla：大肠埃希菌（n = 11）、产酸克雷伯菌（n = 3）和肺炎克雷伯菌（n = 19），而27%的菌株携带bla：大肠埃希菌（n = 7）和肺炎克雷伯菌（n = 20）。
耐碳青霉烯肠杆菌科细菌的患病率为24%，在80%的耐碳青霉烯肠杆菌科细菌中检测到多种碳青霉烯酶基因，其中以bla为主。通过严格的感染控制措施了解我们患者的定植情况可降低碳青霉烯酶水平基因转移的风险。
