A Recombinase Aided Amplification Assay for Rapid Detection of the Carbapenemase Gene and Its Characteristics in .

carbapenemase genes () play an important role in carbapenem-resistant in China. A rapid detection method for genes and investigations into the molecular characteristics of positive were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for was established. This protocol could be completed at 39°C in 15-20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 positive isolates were identified from each sample, of which 23 isolates were . ST11 with was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of positive carbapenem-resistant poses significant challenges for public health control.