The Second Affiliated Hospital of Dalian Medical University, Liaoning, China.
Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Front Cell Infect Microbiol. 2021 Sep 20;11:746325. doi: 10.3389/fcimb.2021.746325. eCollection 2021.
carbapenemase genes () play an important role in carbapenem-resistant in China. A rapid detection method for genes and investigations into the molecular characteristics of positive were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for was established. This protocol could be completed at 39°C in 15-20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 positive isolates were identified from each sample, of which 23 isolates were . ST11 with was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of positive carbapenem-resistant poses significant challenges for public health control.
碳青霉烯酶基因（）在中国耐碳青霉烯类肠杆菌科细菌（）中发挥着重要作用。因此，有必要建立一种快速检测基因的方法，并对阳性菌的分子特征进行研究。本研究建立了一种简便、快速的重组酶辅助扩增检测法（RAA）用于检测。该方案可以在 39°C 下 15-20 分钟内完成。该检测方法的灵敏度为 48 拷贝/反应，特异性为 100%。RAA 方法可用于临床诊断和流行病学调查。在 801 份住院患者粪便样本中，每个样本均鉴定出 34 株阳性分离株，其中 23 株为。ST11 携带 blaNDM-1 是最常见的类型。所有这些菌株均为多重耐药菌，并携带各种毒力基因。耐碳青霉烯类抗生素的肠道携带碳青霉烯酶阳性肠杆菌科细菌对公共卫生控制构成了重大挑战。
