NIOZ Royal Netherlands Institute for Sea Research, Department of Estuarine and Delta Systems, Utrecht University, PO Box 140, 4401 NT, Yerseke, the Netherlands.
Department Ocean Ecosystems, University of Groningen, PO Box 72, 9700 AB, Groningen, the Netherlands.
J Phycol. 2019 Jun;55(3):637-650. doi: 10.1111/jpy.12844. Epub 2019 Apr 1.
Uptake rates of dissolved inorganic phosphorus and dissolved inorganic nitrogen under unsaturated and saturated conditions were studied in young sporophytes of the seaweeds Saccharina latissima and Laminaria digitata (Phaeophyceae) using a "pulse-and-chase" assay under fully controlled laboratory conditions. In a subsequent second "pulse-and-chase" assay, internal storage capacity (ISC) was calculated based on V and the parameter for photosynthetic efficiency F /F . Sporophytes of S. latissima showed a V of 0.80 ± 0.03 μmol · cm · d and a V of 0.30 ± 0.09 μmol · cm · d for dissolved inorganic phosphate (DIP), whereas V for DIN was 11.26 ± 0.56 μmol · cm · d and V was 3.94 ± 0.67 μmol · cm · d . In L. digitata, uptake kinetics for DIP and DIN were substantially lower: V for DIP did not exceed 0.38 ± 0.03 μmol · cm · d while V for DIP was 0.22 ± 0.01 μmol · cm · d . V for DIN was 3.92 ± 0.08 μmol · cm · d and the V for DIN was 1.81 ± 0.38 μmol · cm · d . Accordingly, S. latissima exhibited a larger ISC for DIP (27 μmol · cm ) than L. digitata (10 μmol · cm ), and was able to maintain high growth rates for a longer period under limiting DIP conditions. Our standardized data add to the physiological understanding of S. latissima and L. digitata, thus helping to identify potential locations for their cultivation. This could further contribute to the development and modification of applications in a bio-based economy, for example, in evaluating the potential for bioremediation in integrated multitrophic aquacultures that produce biomass simultaneously for use in the food, feed, and energy industries.
在完全受控的实验室条件下，使用“脉冲-追踪”测定法，研究了在年轻的巨藻（Saccharina latissima）和海带（Laminaria digitata）（褐藻门）孢子体中，溶解无机磷（DIP）和溶解无机氮（DIN）在不饱和和饱和条件下的吸收速率。在随后的第二次“脉冲-追踪”测定中，基于 V 和光合作用效率 F / F 的参数计算了内部储存能力（ISC）。巨藻孢子体的 V 为 0.80±0.03μmol·cm -1·d -1，DIP 的 V 为 0.30±0.09μmol·cm -1·d -1，而 DIN 的 V 为 11.26±0.56μmol·cm -1·d -1，V 为 3.94±0.67μmol·cm -1·d -1。在海带中，DIP 和 DIN 的吸收动力学要低得多：DIP 的 V 不超过 0.38±0.03μmol·cm -1·d -1，而 DIP 的 V 为 0.22±0.01μmol·cm -1·d -1。DIN 的 V 为 3.92±0.08μmol·cm -1·d -1，DIN 的 V 为 1.81±0.38μmol·cm -1·d -1。因此，巨藻对 DIP 的 ISC（27μmol·cm -1）大于海带（10μmol·cm -1），并且在 DIP 限制条件下能够更长时间保持高生长率。我们的标准化数据增加了对巨藻和海带的生理理解，从而有助于确定它们的潜在养殖地点。这有助于开发和改进生物基经济中的应用，例如，在评估同时用于食品、饲料和能源行业的生物量的综合多营养水产养殖中的生物修复潜力方面。
