Abdolahi Sargol, Shokrollahi Borhan, Saadati Nazila, Morammazi Salim
Department of Animal Science, Agriculture School, Sanandaj Branch, Islamic Azad University, Kurdistan, Iran.
Department of Biology, Basic Sciences Faculty, University of Kurdistan, Kurdistan, Iran.
Vet Med Sci. 2019 May;5(2):157-161. doi: 10.1002/vms3.146. Epub 2019 Feb 8.
Melatonin is the main hormone of seasonal breeding in sheep and goat which has an effect on reproductive organs via its receptors. Studies have shown that mutations in melatonin receptor 1A (MTNR1A) gene are related to litter size as well as the ovulation rate in sheep and goats. In this study, polymorphism of two loci in MTNR1A melatonin receptor gene was studied in order to survey their relationship with litter size in Markhoz goats. PCR primers were employed to mask polymorphisms of MTNR1A in 150 does by PCR-RFLP method. After DNA extraction, the PCR-RFLP was performed using Ecol31I and HpaI restriction enzymes. Results showed that these loci were not polymorphic. These results show that the fecundity of Markhoz goats is not linked to MTNR1A. No polymorphism in MTNR1A was found in Markhoz goats, therefore, it is essential to test polymorphism of other genes or loci to facilitate marker-assisted selection techniques to improve reproduction traits in Markhoz goats.
褪黑素是绵羊和山羊季节性繁殖的主要激素，它通过其受体对生殖器官产生影响。研究表明，褪黑素受体1A（MTNR1A）基因的突变与绵羊和山羊的产羔数以及排卵率有关。在本研究中，对MTNR1A褪黑素受体基因中的两个位点的多态性进行了研究，以探究它们与马尔霍兹山羊产羔数的关系。采用PCR引物，通过PCR-RFLP方法检测150只母羊MTNR1A的多态性。提取DNA后，使用Ecol31I和HpaI限制性内切酶进行PCR-RFLP分析。结果表明，这些位点没有多态性。这些结果表明，马尔霍兹山羊的繁殖力与MTNR1A无关。在马尔霍兹山羊中未发现MTNR1A的多态性，因此，有必要检测其他基因或位点的多态性，以促进标记辅助选择技术来改善马尔霍兹山羊的繁殖性状。
