Feng Xingmei, Wang Chenfei, Gu Zhifeng, Ni Jian, Huang Dan, Feng Guijuan, Lian Min, Lu Qi, Song Yihua
1 Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Stomatology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, China.
2 Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Cell Reprogram. 2019 Feb;21(1):18-25. doi: 10.1089/cell.2018.0031.
Rosuvastatin is a synthetic statin of 3-hydroxy-methyl-3-glutamyl coenzyme A reductase inhibitor. It has pleiotropic characteristics including hepatic selectivity, minimal metabolism, inhibition of inflammation, and induction of osteoblast differentiation. In this study, dental pulp stem cells (DPSCs) were treated with lipopolysaccharide alone or with rosuvastatin. Then, we examined the accelerative effects of rosuvastatin on odontoblast differentiation and mineralized nodule formation by real-time polymerase chain reaction (RT-PCR), western blot, alizarin red S staining, and alkaline phosphatase staining. The extent of anti-inflammation was determined by RT-PCR and analysis of the expression of tumor necrosis factor α, interleukin 1β (IL-1β), and IL-6. Furthermore, the activation of nuclear factor kappa B (NF-κB) was determined by western blot. This study demonstrates that rosuvastatin may speed up odontoblast differentiation and rescue inflammatory reaction by suppressing the NF-κB signaling pathway. It is believed that our findings provide novel perceptions on odontogenic differentiation of DPSCs.
瑞舒伐他汀是一种3-羟基-3-甲基戊二酰辅酶A还原酶抑制剂的合成他汀类药物。它具有多效性特征，包括肝脏选择性、最小限度的代谢、炎症抑制以及成骨细胞分化诱导。在本研究中，牙髓干细胞（DPSCs）单独用脂多糖或与瑞舒伐他汀一起处理。然后，我们通过实时聚合酶链反应（RT-PCR）、蛋白质印迹法、茜素红S染色和碱性磷酸酶染色来检测瑞舒伐他汀对成牙本质细胞分化和矿化结节形成的促进作用。通过RT-PCR以及对肿瘤坏死因子α、白细胞介素1β（IL-1β）和IL-6表达的分析来确定抗炎程度。此外，通过蛋白质印迹法确定核因子κB（NF-κB）的激活情况。本研究表明，瑞舒伐他汀可能通过抑制NF-κB信号通路来加速成牙本质细胞分化并挽救炎症反应。相信我们的发现为DPSCs的牙源性分化提供了新的认识。
