Zhang Gan-Lin, Zhang Yi, Cao Ke-Xin, Wang Xiao-Min
Oncology Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University;
Oncology Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University.
J Vis Exp. 2019 Jan 20(143). doi: 10.3791/58604.
A proper animal model is crucial for a better understanding of diseases. Animal models established by different methods (subcutaneous injections, xenografts, genetic manipulation, chemical reagents induction, etc.) have various pathological characters and play important roles in investigating certain aspects of diseases. Although no single model can totally mimic the whole human disease progression, orthotopic organs disease models with a proper stromal environment play an irreplaceable role in understanding diseases and screening for potential drugs. In this article, we describe how to implant breast cancer cells into the mammary fat pad in a simple, less invasive, and easy-to-handle way, and follow the metastasis to distant organs. With the proper features of primary tumor growth, breast and nipple pathological changes, and a high occurrence of other organs' metastasis, this model maximumly mimics human breast cancer progression. Primary tumor growth in situ, long-distant metastasis, and the tumor microenvironment of breast cancer can be investigated by using this model.
合适的动物模型对于更好地理解疾病至关重要。通过不同方法（皮下注射、异种移植、基因操作、化学试剂诱导等）建立的动物模型具有各种病理特征，在研究疾病的某些方面发挥着重要作用。尽管没有单一模型能够完全模拟整个人类疾病进程，但具有合适基质环境的原位器官疾病模型在理解疾病和筛选潜在药物方面发挥着不可替代的作用。在本文中，我们描述了如何以简单、微创且易于操作的方式将乳腺癌细胞植入乳腺脂肪垫，并追踪其向远处器官的转移。该模型具有原发性肿瘤生长、乳腺和乳头病理变化以及其他器官高转移发生率等合适特征，最大程度地模拟了人类乳腺癌进程。利用该模型可以研究原发性肿瘤原位生长、远距离转移以及乳腺癌的肿瘤微环境。
