Sugimoto Hiroki, Kawakami Kiyoshi
Division of Biology, Center for Molecular Medicine, Jichi Medical University.
Division of Biology, Center for Molecular Medicine, Jichi Medical University;
J Vis Exp. 2019 Jan 23(143). doi: 10.3791/59027.
Gait disturbance is frequently observed in patients with movement disorders. In mouse models used for movement disorders, gait analysis is important behavioral test to determine whether the mice mimic the symptoms of patients. Motor deficits are often induced by stress when no spontaneous motor phenotype is observed in the mouse models. Therefore, gait analysis followed by stress loading would be a sensitive method for evaluating the motor phenotype in mouse models. However, researchers face the requirement of an expensive apparatus to obtain quantitative results automatically from gait analysis. For stress, stress loading by simple methods without expensive apparatuses required for electric shock and forced running is desirable. Therefore, we introduce a simple and low-cost protocol consisting of footprint analysis with paper and ink, hanging box test to evaluate motor function, and stress loading defined by restraint with a conical tube. The motor deficits of mice were successfully detected by this protocol.
步态障碍在运动障碍患者中经常出现。在用于研究运动障碍的小鼠模型中，步态分析是确定小鼠是否模拟患者症状的重要行为测试。当在小鼠模型中未观察到自发运动表型时，运动缺陷通常由应激诱导。因此，在应激负荷后进行步态分析将是评估小鼠模型运动表型的一种敏感方法。然而，研究人员面临着需要昂贵设备才能从步态分析中自动获得定量结果的问题。对于应激，理想的是通过简单方法进行应激负荷，而无需电击和强制跑步所需的昂贵设备。因此，我们介绍一种简单且低成本的方案，该方案包括用纸张和墨水进行足迹分析、用于评估运动功能 的悬挂箱测试，以及由锥形管约束定义的应激负荷。通过该方案成功检测到了小鼠的运动缺陷。
