School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health , Jinan University , Guangzhou 510632 , China.
Department of Biological Sciences , University of Alberta , Edmonton T6G 2R3 , Alberta Canada.
J Agric Food Chem. 2019 Mar 6;67(9):2429-2436. doi: 10.1021/acs.jafc.8b05373. Epub 2019 Feb 20.
Agricultural use of neonicotinoid insecticides is increasing worldwide, posing a risk to nontarget organisms. The present study investigated developmental toxicity of a widely used neonicotinoid, acetamiprid, to zebrafish embryos. Sublethal (malformations, hatchability, heart rate, body length, alteration of spontaneous movement and touch responses) and lethal effects were monitored during exposure period from 6 h post fertilization (hpf) to 120 hpf. Zebrafish embryos exhibited significant mortality and teratogenic effects at acetamiprid concentration greater than 263 mg/L, with bent spine being the main malformation. Toxicity spectra were constructed to rank the sensitivity of individual end points to acetamiprid exposure and impaired spontaneous movement was the most sensitive end point of those tested. The present study provides the basis for understanding developmental toxicity of acetamiprid exposure to zebrafish embryos. This information is critical for future studies evaluating aquatic risk from neonicotinoids as little is known regarding adverse effects of neonicotinoids to aquatic vertebrate species.
农业中越来越多地使用新烟碱类杀虫剂，这对非靶标生物构成了风险。本研究调查了一种广泛使用的新烟碱类杀虫剂——乙酰甲胺磷对斑马鱼胚胎的发育毒性。在从受精后 6 小时（hpf）到 120 hpf 的暴露期间，监测了亚致死（畸形、孵化率、心率、体长、自发运动和触摸反应的改变）和致死效应。在乙酰甲胺磷浓度大于 263 mg/L 时，斑马鱼胚胎表现出显著的死亡率和致畸作用，弯曲的脊柱是主要的畸形。构建了毒性谱，以对各个终点对乙酰甲胺磷暴露的敏感性进行排序，并且受影响的自发运动是测试的最敏感终点之一。本研究为了解乙酰甲胺磷暴露对斑马鱼胚胎的发育毒性提供了基础。这些信息对于未来评估新烟碱类对水生脊椎动物物种的水生风险的研究至关重要，因为人们对新烟碱类对水生脊椎动物物种的不良影响知之甚少。
