Babic V, Datla R S, Scoles G J, Keller W A
Department of Crop Science and Plant Ecology, University of Saskatchewan, 51 Campus Dr., Saskatoon, Saskatchewan, S7N 5A8, Canada, , , , , , CA.
Plant Biotechnology Institute, National Research Council, 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Fax no.: +1-306-975-4839 E-mail:
Plant Cell Rep. 1998 Jan;17(3):183-188. doi: 10.1007/s002990050375.
Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30-50% of the explants producing GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots but at a lower frequency (1-2%).
从埃塞俄比亚芥的子叶柄和下胚轴很容易实现芽器官发生和植株再生。这些外植体用于农杆菌介导的转化。一个含有选择标记基因新霉素磷酸转移酶II、草丁膦乙酰转移酶和报告基因β-葡萄糖醛酸酶的构建体，在串联35S启动子的控制下，用于转化。虽然子叶柄和下胚轴都实现了转化，但子叶柄反应最佳，在25毫克/升卡那霉素上选择后，30%-50%的外植体产生了GUS阳性芽。直接在L-草丁膦上选择也产生了抗性芽，但频率较低（1%-2%）。
