Zhu Li-Hua, Krens Frans, Smith Mark A, Li Xueyuan, Qi Weicong, van Loo Eibertus N, Iven Tim, Feussner Ivo, Nazarenus Tara J, Huai Dongxin, Taylor David C, Zhou Xue-Rong, Green Allan G, Shockey Jay, Klasson K Thomas, Mullen Robert T, Huang Bangquan, Dyer John M, Cahoon Edgar B
Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, SE 230 53, Alnarp, Sweden.
Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
Sci Rep. 2016 Feb 26;6:22181. doi: 10.1038/srep22181.
Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.
从聚合物到润滑剂等工业应用的原料大多来自石油，而石油是一种不可再生资源。具有为特定工业用途量身定制的脂肪酸结构和储存形式的植物油，提供了石化类功能的可再生且具有潜在可持续性的来源。通过生物技术可以生产出各种各样的工业植物油，但可能需要专门用于特种油生产的非商品油籽平台才能被商业接受。在这里，我们展示了三种十字花科油籽——海甘蓝、亚麻荠和油菜，它们都没有被广泛种植用于食品，作为用于润滑剂应用的蜡酯复杂代谢工程的宿主的可行性。每种作物都培育出了蜡酯含量超过种子油总量20%的品系，并通过下调脂肪酸多不饱和程度进一步提高了高温氧化稳定性。对优化后的产蜡酯海甘蓝进行田间种植，证明了这些工程作物的商业实用性，以及在专用特种油籽中可持续生产其他工业油的途径。
