Larronde F, Krisa S, Decendit A, Chèze C, Deffieux G, Mérillon J M
Groupe d'Etude des Substances Naturelles à Intérêt Thérapeutique, EA 491, Faculté de Pharmacie, Université de Bordeaux II, 3 place de la Victoire, F-33000 Bordeaux, France Fax: +33-5-56917988 e-mail:
Plant Cell Rep. 1998 Sep;17(12):946-950. doi: 10.1007/s002990050515.
Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 M sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars were required for the induction of polyphenol accumulation. Indeed, the polyols, mannitol and sorbitol, had no effect on this accumulation. We established a model system to investigate the mechanism of sucrose regulation of polyphenol production without inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with the increase in anthocyanin accumulation in grape cells.
研究发现，蔗糖可调节葡萄细胞培养物中多酚的积累。添加0.15 M蔗糖后，花青素的产量增加了12倍，而芪类化合物的产量仅受到轻微影响。蔗糖并未起到物理作用，因为诱导多酚积累需要代谢糖。事实上，多元醇、甘露醇和山梨醇对这种积累没有影响。我们建立了一个模型系统，以研究蔗糖调节多酚生成的机制，同时不抑制葡萄细胞生长。向培养基中添加蔗糖后，葡萄细胞中积累的主要糖类是葡萄糖和果糖，达到干重的40%。这些己糖水平的增加与葡萄细胞中花青素积累的增加密切相关。
