Food Research and Development Centre, Agriculture Canada, 3600 Casavant Boulevard West, J2S 8E3, St-Hyacinthe, Québec, Canada.
Plant Cell Rep. 1990 Jul;9(3):143-6. doi: 10.1007/BF00232091.
A cell suspension of grape, Vitis vinifera L. cv Gamay Fréaux, was grown under different conditions of water stress (high external osmotic potential) induced by an increase of sucrose concentration or by the addition of mannitol to the culture medium. Best growth (cell density) was achieved in the low osmotic potential medium. Increasing the osmotic potential of the medium from -0.5 MPa to -0.9 MPa medium resulted in a significant increase in accumulation of anthocyanins in pigmented cells. Regulation of the osmotic potential of culture medium may be useful in controlling anthocyanin production.
采用增加蔗糖浓度或在培养液中添加甘露醇的方法来诱导葡萄（Vitis vinifera L. cv Gamay Fréaux）悬浮细胞产生不同程度的水分胁迫（高渗透压势），在不同的胁迫条件下培养细胞悬浮液。在低渗透压势的培养液中细胞生长（细胞密度）最佳。培养液的渗透压势从-0.5 MPa 增加到-0.9 MPa 时，有色细胞中花色素苷的积累明显增加。调节培养液的渗透压势可能有助于控制花色素苷的产生。
