东部类蜀黍（Tripsacum dactyloides L.）诱导四倍体的染色体加倍及繁殖方式

Chromosome doubling and mode of reproduction of induced tetraploids of eastern gamagrass (Tripsacum dactyloides L.).

作者信息

Salon P R, Earle E D

机构信息

USDA NRCS Big Flats Plant Materials Center RD1, Box 360A Rt 352, Corning, NY 14830, USA Fax: +1-607-5628516, , , , , , US.

Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, Ithaca, NY 14853-1901, USA, , , , , , US.

出版信息

Plant Cell Rep. 1998 Aug;17(11):881-885. doi: 10.1007/s002990050502.

DOI:10.1007/s002990050502

PMID:30736561

Abstract

Eastern gamagrass, (Tripsacum dactyloides L.) is a perennial, warm-season grass that is being developed as a forage plant. Shoots were derived from callus initiated from immature embryos and immature inflorescences of diploid (2n=2x=36) gynomonoecious eastern gamagrass. These shoots were induced to microtiller in the presence of 3 mg/l benzyladenine. Amiprophosmethyl (10, 15, or 20 μM) was applied to 27 microtillers for 3-5 days to induce chromosome doubling. All 14 surviving plants were tetraploid, (2n=4x=72), as determined by flow cytometry or chromosome counts. These plants were morphologically normal and produced seed. Test crosses were made with a known diploid. Flow cytometry and chromosome counts showed that the progeny were triploid, proving that the induced tetraploids reproduce sexually.

摘要

东部伽马草（Tripsacum dactyloides L.）是一种多年生暖季型禾本科植物，正被开发用作饲料作物。芽源自二倍体（2n = 2x = 36）雌雄同株的东部伽马草未成熟胚和未成熟花序诱导产生的愈伤组织。在含有3 mg/l苄氨基腺嘌呤的条件下，这些芽被诱导产生微型分蘖。将氨丙膦酸甲酯（10、15或20 μM）施用于27个微型分蘖3 - 5天以诱导染色体加倍。通过流式细胞术或染色体计数确定，所有14株存活植株均为四倍体（2n = 4x = 72）。这些植株形态正常且能产生种子。用已知的二倍体进行了测交。流式细胞术和染色体计数表明后代为三倍体，证明诱导产生的四倍体能够进行有性繁殖。