Mohapatra T, Kirti P B, Dinesh Kumar V, Prakash S, Chopra V L
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110012, India e-mail:
Plant Cell Rep. 1998 Jul;17(10):814-818. doi: 10.1007/s002990050489.
Detailed molecular analysis of the somatic hybrid plants of Diplotaxis catholica+B. juncea indicated random chloroplast segregation. One of the five hybrid plants analyzed derived its chloroplasts from D. catholica and two hybrids had chloroplasts of B. juncea origin. Two hybrid plants maintained mixed population of chloroplasts. The mitochondrial (mt) genomes of the fusion partners had undergone recombinations. Occurrence of fragments specific to both the parents in HindIII digestion followed by atp 9 probing, as in hybrid DJ5, provided evidence for intergenomic mitochondrial recombination between D. catholica and B. juncea. Similar mt genome organization in two hybrids (DJ3 and DJ6) suggested that intergenomic recombination may be preferred at specific sites. Hybrid DJ1 had about 70% similarity to D. catholica in mt genome organization. mt genomes of hybrids DJ2, 3, 5, and 6 differed from B. juncea by 14.3-28%. The significance of these novel mt genome organizations in developing novel male sterility systems is discussed.
对海滨双角草+芥菜型油菜体细胞杂种植物的详细分子分析表明叶绿体随机分离。在分析的五株杂种植物中，有一株的叶绿体来自海滨双角草，两株杂种的叶绿体源自芥菜型油菜。两株杂种植物维持着叶绿体的混合群体。融合亲本的线粒体（mt）基因组发生了重组。如在杂种DJ5中，经HindIII酶切后再用atp 9探针检测，出现了双亲特有的片段，这为海滨双角草和芥菜型油菜之间的基因组间线粒体重组提供了证据。两个杂种（DJ3和DJ6）中相似的线粒体基因组组织表明基因组间重组可能在特定位点更易发生。杂种DJ1的线粒体基因组组织与海滨双角草有大约70%的相似性。杂种DJ2、3、5和6的线粒体基因组与芥菜型油菜的差异为14.3 - 28%。本文讨论了这些新型线粒体基因组组织在开发新型雄性不育系统中的意义。
