Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India.
Plant Cell Rep. 1995 Jun;14(9):593-7. doi: 10.1007/BF00231945.
Intergeneric somatic hybrids Diplotaxis catholica (2n=18) + Brassica juncea (2n=36) were produced by fusing mesophyll protoplasts of the former and hypocotyl protoplasts of the latter using polyethylene glycol. Out of 52 somatic embryos, 24 produced plants of intermediate morphology. Cytological analysis of 16 plants indicated that 15 were symmetric hybrids carrying 54 chromosomes, the sum of the parental chromosome numbers. One hybrid was asymmetric with 45 chromosomes. Nuclear hybridity of five putative hybrids was confirmed by the Southern hybridization pattern of full length 18s-25s wheat nuclear rDNA probe which revealed the presence of Hind III fragments characteristic of both the parental species. The hybridization pattern of mitochondria specific gene probe cox I indicated that three of the hybrids carried B. juncea mitochondria and one carried mitochondria of D. catholica. Presence of novel 3.5 kb Hind III and 4.8 kb Bgl II fragments suggested the occurrence of mtDNA recombination in one of the hybrids. The hybrids were pollen sterile. However, seeds were obtained from most of the hybrids by back crossing with B. juncea.
通过聚乙二醇融合前者的叶肉原生质体和后者的下胚轴原生质体，产生了芥菜（2n=18）+芸薹属（2n=36）属间体细胞杂种。在 52 个体细胞胚中，有 24 个产生了中间形态的植物。对 16 株植物的细胞学分析表明，其中 15 株为携带 54 条染色体的对称杂种，是双亲染色体数目的总和。一株杂种为不对称杂种，有 45 条染色体。通过全长 18s-25s 小麦核 rDNA 探针的 Southern 杂交模式证实了五个假定杂种的核杂种性，该探针显示出亲本种特有的 Hind III 片段。线粒体特异性基因探针 cox I 的杂交模式表明，其中 3 个杂种携带芸薹属的线粒体，而 1 个杂种携带芥菜的线粒体。新型 3.5kb Hind III 和 4.8kb Bgl II 片段的存在表明，其中一个杂种发生了 mtDNA 重组。杂种花粉不育。然而，通过与芸薹属回交，大多数杂种都获得了种子。
