Xu J F, Liu C B, Han A M, Feng P S, Su Z G
Institute of Biochemical Engineering, Dalian University of Technology, Dalian, 116012, China Fax no.: +86-0411-3633080, , , , , , CN.
Plant Cell Rep. 1998 Feb;17(4):288-293. doi: 10.1007/s002990050394.
Strategies of elicitation and precursor feeding were applied to improve salidroside production in cell suspension cultures of Rhodiola sachalinensis. Of the seven elicitors examined, that extracted from Aspergillus niger was the most effective, increasing the salidroside content by five-fold when added on the day of inoculation 40 mg carbohydrate is medium. Three possible precursors for salidroside synthesis, L-phenylalanine, L-tyrosol and L-tyrosine were added to the cultures. A high content of salidroside (1.440%) was attained with an initial L-tyrosol concentration of 0.5 mM in the medium. Combined application of the two strategies resulted in a significantly high salidroside content of 1.734%, corresponding to a salidroside yield of 200 mg/l.
采用诱导和前体饲喂策略提高高山红景天细胞悬浮培养中红景天苷的产量。在所检测的7种诱导子中，从黑曲霉中提取的诱导子效果最佳，在接种当天添加40 mg碳水化合物/培养基时，红景天苷含量增加了5倍。向培养物中添加了红景天苷合成的3种可能前体，即L-苯丙氨酸、L-酪醇和L-酪氨酸。培养基中初始L-酪醇浓度为0.5 mM时，红景天苷含量较高（1.440%）。两种策略联合应用使红景天苷含量显著提高至1.734%，相当于红景天苷产量为200 mg/l。
