Li X Y, Huang F H, Gbur E E
Department of Horticulture, 316 Plant Science Building, University of Arkansas, Fayetteville, AR 72701, USA Fax no.: +1-501-575-8619, , , , , , US.
Agricultural Statistics Laboratory, 101 Agricultural Annex, University of Arkansas, Fayetteville, AR 72701, USA, , , , , , US.
Plant Cell Rep. 1998 Feb;17(4):298-301. doi: 10.1007/s002990050396.
Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM basal medium proved superior to DCR and LP (LP basal salts plus BM organic nutrients). No extrusion from megagametophytes was exhibited on LP medium. The combination of 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l 6-benzylaminopurine (BA) resulted in a higher extrusion frequency than that of 11 mg/l 2,4-D, 4.5 mg/l BA and 4.3 mg/l kinetin. Phytagel at 1 g/l resulted in the highest explant browning, but the lowest extrusion frequency, while 4 g/l Phytagel induced some dry embryogenic extrusions. Phytagel at 2 g/l was regarded as the best level for initiation of embryogenic cultures.
低起始频率是将体细胞胚胎发生应用于松树物种克隆生产的主要障碍之一。在火炬松（Pinus taeda L.）中，已经研究了影响起始的因素，包括基本培养基、植物生长调节剂和植物凝胶浓度。BM基本培养基被证明优于DCR和LP（LP基本盐加BM有机营养物）。在LP培养基上未观察到雌配子体有外植体挤出。3 mg/l 2,4-二氯苯氧乙酸（2,4-D）和0.5 mg/l 6-苄基腺嘌呤（BA）的组合比11 mg/l 2,4-D、4.5 mg/l BA和4.3 mg/l激动素产生更高的外植体挤出频率。1 g/l的植物凝胶导致外植体褐化程度最高，但挤出频率最低，而4 g/l的植物凝胶诱导了一些干燥的胚性外植体挤出。2 g/l的植物凝胶被认为是启动胚性培养的最佳水平。
