蝴蝶兰的愈伤组织诱导和体细胞胚胎发生

Callus induction and somatic embryogenesis of Phalaenopsis.

作者信息

Ishii Y, Takamura T, Goi M, Tanaka M

机构信息

Department of Horticulture, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan, , , , , , JP.

出版信息

Plant Cell Rep. 1998 Apr;17(6-7):446-450. doi: 10.1007/s002990050423.

DOI:10.1007/s002990050423

PMID:30736617

Abstract

Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l. Medium containing 200 ml ⋅ l coconut water together with 40 g ⋅ l sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.

摘要

对蝴蝶兰‘理查德·谢弗’‘圣克鲁兹’通过体细胞胚胎发生进行愈伤组织诱导和植株再生进行了研究。原球茎状茎段在含有蔗糖的瓦欣和温特培养基中形成愈伤组织。蔗糖的最佳浓度为40 g·L。含有200 mL·L椰子水和40 g·L蔗糖的培养基对愈伤组织诱导有效。对于愈伤组织诱导，结冷胶比琼脂更适合作为凝胶剂。愈伤组织转移到不含蔗糖的培养基后很容易形成原球茎。组织学观察表明，原球茎是体细胞胚。通过体细胞胚胎发生再生的开花植株未观察到变异。