Muhitch M J, Shatters R G
Mycotoxin Research Unit, USDA, ARS, National Center for Agricultural Utilization Research, 1815 N. University St., Peoria, IL 61604, USA, , , , , , US.
USDA/ARS at the Department of Agronomy, University of Florida, Gainesville, FL 32605, USA, , , , , , US.
Plant Cell Rep. 1998 Apr;17(6-7):476-481. doi: 10.1007/s002990050428.
Kernel culture was assessed for evaluating novel gene expression in developing maize (Zea mays L.) seeds by comparing the transient expression of maize ubiquitin (Ubi-1) promoter-driven β-glucuronidase (GUS) delivered by particle bombardment in kernels grown in culture with those grown in planta. With kernels from either source, GUS expression, as determined by histochemical staining, was widespread in young, actively growing kernels, but it diminished with kernel age and by 25 days after pollination was found only in the embryo. Transient expression of Ubi-1 in kernels grown in vitro was not affected by wounding, ethylene treatment, pathogen invasion, or heat shock. In contrast, the plant hormones indole-3-acetic acid and kinetin both stimulated transient Ubi-1 expression in the endosperm, particularly at the periphery. Transient gene expression in developing maize seeds grown in vitro should allow for facile and rapid evaluation of the tissue-specificity and environmental responses of novel gene constructs in developing maize seeds.
通过比较微粒轰击传递的玉米泛素（Ubi-1）启动子驱动的β-葡萄糖醛酸酶（GUS）在离体培养生长的玉米粒和在植株上生长的玉米粒中的瞬时表达，评估了核心培养物用于评价发育中的玉米（Zea mays L.）种子中新型基因的表达情况。对于来自任一来源的玉米粒，通过组织化学染色确定的GUS表达在幼嫩、生长活跃的玉米粒中广泛存在，但随着玉米粒成熟而减少，并且在授粉后25天时仅在胚中发现。体外生长的玉米粒中Ubi-1的瞬时表达不受创伤、乙烯处理、病原体入侵或热激的影响。相反，植物激素吲哚-3-乙酸和激动素均刺激胚乳中Ubi-1的瞬时表达，特别是在胚乳周边。体外生长的发育中的玉米种子中的瞬时基因表达应有助于快速简便地评估新型基因构建体在发育中的玉米种子中的组织特异性和环境响应。
