Hammatt N, Grant N J
Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK, , , , , , GB.
Plant Cell Rep. 1998 Apr;17(6-7):526-530. doi: 10.1007/s002990050436.
Leaves excised from shoot cultures of Prunus avium cvs. F12/1 and Charger and genotype 1908, and from five genotypes of P. serotina and two hybrids of P. avium×P. sargentii developed shoots on Woody Plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Regeneration in both P. avium 1908 and a genotype of P. serotina was improved using TDZ rather than BA in the medium. Regeneration occurred more frequently in P. serotina if leaves were cultured on medium with WPM rather than modified Driver and Kuniyuki walnut medium. The proportions of leaves that regenerated varied between genotypes of the same species. Regenerated shoots of both P. avium and P. serotina developed into shoot cultures following transfer to the media used to produce the shoot cultures used as explant sources.
从欧洲甜樱桃品种F12/1、“充电器”和基因型1908的茎段培养物上切取的叶片，以及从五种黑樱桃基因型和两种欧洲甜樱桃×萨金特樱桃杂种上切取的叶片，在添加了苄基腺嘌呤（BA）或噻苯隆（TDZ）的木本植物培养基（WPM）上形成了芽。在培养基中使用TDZ而非BA时，欧洲甜樱桃1908和一种黑樱桃基因型的再生能力得到了提高。如果将叶片培养在含有WPM的培养基上而非改良的Driver和Kuniyuki核桃培养基上，黑樱桃的再生更为频繁。同一物种不同基因型之间再生叶片的比例有所不同。欧洲甜樱桃和黑樱桃的再生芽在转移到用于产生用作外植体来源的茎段培养物的培养基后，发育成了茎段培养物。
