Matt Andrea, Jehle Johannes A
Laboratory for Biotechnological Crop Protection, Department of Phytopathology, Agricultural Service Center Palatinate, 67435 Neustadt an der Weinstrasse, Germany.
Plant Cell Rep. 2005 Oct;24(8):468-76. doi: 10.1007/s00299-005-0964-6. Epub 2005 Jun 10.
Regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. "Schneiders", "Sweetheart", "Starking Hardy Giant", "Kordia" and "Regina" (Prunus avium L.). The influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. Both, DKW/WPM (1:1) and Quoirin/Lepoivre (QL) basal media stimulated organogenesis more than QL/WPM (1:1), Chee and Pool (CP), Murashige Skoog (MS), Driver and Kuniyuki (DKW) or woody plant (WPM) media did. An induction phase in darkness resulted in lower or zero regeneration rates. The best regeneration efficiencies were generally obtained with thidiazuron in combination with indole-3-butyric-acid. The addition of silver thiosulfate resulted in a similar or reduced regeneration efficiency. Significant genotypic variability in adventitious bud formation was evident for both explant sources, leaf and internode section. Adventitious shoots were obtained from 11% of leaf explants and 50% of internode sections indicating that shoot regeneration from internodes was significantly more efficient than from leaves.
对五个经济上重要的甜樱桃品种，即“施奈德”、“甜心”、“斯塔克硬粒大樱桃”、“科迪亚”和“瑞吉娜”（欧洲甜樱桃）的叶片不定芽再生以及首次从节间切段的不定芽再生进行了比较和优化。评估了基本培养基、碳源、植物激素的组合和用量、乙烯抑制剂如硫代硫酸银以及16小时光照：8小时黑暗的光周期与完全黑暗的影响。DKW/WPM（1:1）和夸林/勒普瓦尔（QL）基本培养基比QL/WPM（1:1）、奇和普尔（CP）、Murashige Skoog（MS）、德赖弗和邦行（DKW）或木本植物（WPM）培养基更能刺激器官发生。黑暗中的诱导阶段导致再生率较低或为零。通常使用噻二唑隆与吲哚-3-丁酸组合可获得最佳再生效率。添加硫代硫酸银导致再生效率相似或降低。对于叶片和节间切段这两种外植体来源，不定芽形成存在显著的基因型差异。从11%的叶片外植体和50%的节间切段获得了不定芽，这表明从节间再生芽比从叶片再生芽效率显著更高。
