Amor M B, Guis M, Latché A, Bouzayen M, Pech J-C, Roustan J-P
Ecole Nationale Supérieure Agronomique de Toulouse, UA INRA, 145 avenue de Muret, 31076 Toulouse cedex, France Fax no.: +33-5-62-13-65-41 E-mail:
Plant Cell Rep. 1998 Apr;17(6-7):586-589. doi: 10.1007/s002990050447.
The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 µM 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.
利用表达反义1-氨基环丙烷-1-羧酸（ACC）氧化酶基因的转基因甜瓜植株，研究了乙烯在芽再生中的作用。ACC氧化酶催化乙烯生物合成的最后一步。转基因植株的叶片和子叶外植体表现出较低的ACC氧化酶活性和乙烯产量，而与未转化的对照组织相比，这些组织的再生能力大大提高（分别提高了3.5倍和2.8倍）。添加由50或100 µM 2-氯乙基膦酸释放的乙烯显著降低了转基因组织的芽再生率。结果清楚地表明，乙烯在甜瓜离体形态发生中起重要作用。
