Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, 010021, Hohhot, People's Republic of China.
Biotechnol Lett. 2011 Jan;33(1):55-61. doi: 10.1007/s10529-010-0398-2. Epub 2010 Sep 24.
Melons have short shelf-lives due to fruit ripening caused by ethylene production. The 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene is essential for ethylene biosynthesis. As fruit ripening in other fruit crops can be deterred by down-regulation of ACC oxidase expression, we have carried out similar work to improve fruit quality and shelf-life of the melon Cucumis melo. A marker-free and vector-free antisense 1-aminocyclopropane-1-carboxylic acid oxidase construct was transformed into melon via the pollen-tube pathway. Based on phenotype analysis together with RT-PCR data, a transformation frequency of 0.7% was achieved. The transgenic fruits showed respiration rate and endogenous ethylene production level at approx. 15 and 6% of those of wild-type fruits, respectively. These fruits also demonstrated improved flesh firmness and exhibited extended shelf-life of 30 days compared to less than 12 days for the wild type fruits.
由于乙烯生产导致果实成熟，瓜类的货架期很短。1-氨基环丙烷-1-羧酸（ACC）氧化酶基因是乙烯生物合成所必需的。由于其他水果作物的果实成熟可以通过下调 ACC 氧化酶的表达来阻止，我们已经进行了类似的工作，以提高甜瓜的果实品质和货架期。我们通过花粉管途径将无标记和无载体的反义 1-氨基环丙烷-1-羧酸氧化酶构建体转化到瓜类中。基于表型分析和 RT-PCR 数据，转化频率达到了 0.7%。转基因果实的呼吸速率和内源乙烯产生水平分别约为野生型果实的 15%和 6%。与野生型果实的 12 天以下相比，这些果实的果肉硬度也得到了改善，货架期延长至 30 天。
