Process and Systems Engineering Center (PROSYS), Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, 2800 Kgs. Lyngby, Denmark.
Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark.
Trends Biotechnol. 2019 Jul;37(7):697-706. doi: 10.1016/j.tibtech.2019.01.002. Epub 2019 Feb 5.
Miniaturized stirred bioreactors (MSBRs) are gaining popularity as a cost-effective approach to scale-down experimentation. However, realizing conditions that reflect the large-scale process accurately can be challenging. This article highlights common challenges of using MSBRs for scale-down. The fundamental difference between oxygen mass transfer coefficient (ka) and oxygen transfer rate scaling is addressed and the difficulty of achieving turbulent flow and industrially relevant tip speeds is described. More practical challenges of using MSBR systems for scale-down are also discussed, including the risk of vortex formation, changed volume dynamics, and wall growth. By highlighting these challenges, the article aims to create more awareness of these difficulties and to contribute to improved design of scale-down experiments.
微型搅拌式生物反应器（MSBR）作为一种降低成本的实验方法，正日益受到关注。然而，实现准确反映大规模工艺的条件具有挑战性。本文强调了使用 MSBR 进行缩小规模实验的常见挑战。本文还讨论了使用 MSBR 系统进行缩小规模的其他更实际的挑战，包括涡旋形成的风险、改变的体积动态和壁生长。通过强调这些挑战，本文旨在提高对这些困难的认识，并有助于改进缩小规模实验的设计。
